Pseudomonas aeruginosa PAO1, PA3988 (lptE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3988
Name
lptE
Replicon chromosome
Genomic location 4469613 - 4470236 (+ strand)

Cross-References

RefSeq NP_252677.1
GI 15599183
Affymetrix PA3988_at
DNASU PaCD00007741
Entrez 878911
GenBank AAG07375.1
INSDC AAG07375.1
NCBI Locus Tag PA3988
protein_id(GenBank) gb|AAG07375.1|AE004816_11|gnl|PseudoCAP|PA3988
TIGR NTL03PA03988
UniParc UPI00000C5BB2
UniProtKB Acc Q9HX32
UniProtKB ID Q9HX32_PSEAE
UniRef100 UniRef100_W0YY23
UniRef50 UniRef50_S3MKJ9
UniRef90 UniRef90_A6V0C1

Product

Feature Type CDS
Coding Frame 1
Product Name
LPS-assembly lipoprotein LptE
Product Name Confidence: Class 2
Synonyms hypothetical protein
Evidence for Translation
Detected using LC-MS/MS (22.88 kDa, PMID:21751344)
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.2, molecular weight:22.9, PMID:21674802)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using 2-DE, MALDI-TOF/MS analysis (22.88 kDa, pI 5.2, PMID:16335974)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -4.55
Kyte-Doolittle Hydrophobicity Value -0.448
Molecular Weight (kDa) 22.9
Isoelectric Point (pI) 4.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 1
Unknown Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization
Detected in sarcosine-insoluble outer membrane fraction using 2-DE, MALDI-TOF/MS analysis (PMID:16335974)

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5IVA TRANSPORT PROTEIN 03/20/16 The LPS Transporter LptDE from Pseudomonas aeruginosa, core complex Pseudomonas aeruginosa 2.988 X-RAY DIFFRACTION 100.0
2N8X LIPID BINDING PROTEIN 10/28/15 Solution structure of LptE from Pseudomonas Aerigunosa Pseudomonas aeruginosa PAO1 NOT SOLUTION NMR 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 60 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001693 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3988
Search term: lptE

Human Homologs

References

Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Structural basis for lipopolysaccharide insertion in the bacterial outer membrane.
Qiao S, Luo Q, Zhao Y, Zhang XC, Huang Y
Nature 2014 Jul 3;511(7507):108-11
PubMed ID: 24990751