Pseudomonas aeruginosa PAO1, PA3992 (sltB3)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3992
Name
sltB3
Synonym: soluble lytic (exolytic) transglycosylase
Replicon chromosome
Genomic location 4472131 - 4473477 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252681.1
GI 15599187
Affymetrix PA3992_at
Entrez 878935
GenBank AAG07379.1
INSDC AAG07379.1
NCBI Locus Tag PA3992
protein_id(GenBank) gb|AAG07379.1|AE004817_3|gnl|PseudoCAP|PA3992
TIGR NTL03PA03992
UniParc UPI00000C5BB6
UniProtKB Acc Q9HX28
UniProtKB ID Q9HX28_PSEAE
UniRef100 UniRef100_Q9HX28
UniRef50 UniRef50_W9V004
UniRef90 UniRef90_Q9HX28

Product

Feature Type CDS
Coding Frame 1
Product Name
SltB3
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (47.76 kDa, PMID:21751344)
Charge (pH 7) 2.87
Kyte-Doolittle Hydrophobicity Value -0.216
Molecular Weight (kDa) 47.8
Isoelectric Point (pI) 8.22

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5AO8 TRANSFERASE 09/09/15 Crystal Structure of SltB3 from Pseudomonas aeruginosa in complex with NAG-NAM-pentapeptide PSEUDOMONAS AERUGINOSA 2.23 X-RAY DIFFRACTION 99.8
5ANZ HYDROLASE 09/09/15 Crystal Structure of SltB3 from Pseudomonas aeruginosa. PSEUDOMONAS AERUGINOSA 1.614 X-RAY DIFFRACTION 100.0
5AO7 TRANSFERASE 09/09/15 Crystal Structure of SltB3 from Pseudomonas aeruginosa in complex with NAG-anhNAM-pentapeptide PSEUDOMONAS AERUGINOSA 2.09 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 226 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001689 (532 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3992
Search term: sltB3
Search term: SltB3

Human Homologs

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Characterization of soluble and membrane-bound family 3 lytic transglycosylases from Pseudomonas aeruginosa.
Blackburn NT, Clarke AJ
Biochemistry 2002 Jan 22;41(3):1001-13
PubMed ID: 11790124