Pseudomonas aeruginosa PAO1, PA3992 (sltB3)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3992
Name
sltB3
Synonym: soluble lytic (exolytic) transglycosylase
Replicon chromosome
Genomic location 4472131 - 4473477 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_252681.1
GI 15599187
Affymetrix PA3992_at
Entrez 878935
GenBank AAG07379.1
INSDC AAG07379.1
NCBI Locus Tag PA3992
protein_id(GenBank) gb|AAG07379.1|AE004817_3|gnl|PseudoCAP|PA3992
TIGR NTL03PA03992
UniParc UPI00000C5BB6
UniProtKB Acc Q9HX28
UniProtKB ID Q9HX28_PSEAE
UniRef100 UniRef100_Q9HX28
UniRef50 UniRef50_W9V004
UniRef90 UniRef90_Q9HX28

Product

Feature Type CDS
Coding Frame 1
Product Name
SltB3
Product Name Confidence: Class 1
Evidence for Translation
Detected using LC-MS/MS (47.76 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.87
Kyte-Doolittle Hydrophobicity Value -0.216
Molecular Weight (kDa) 47.8
Isoelectric Point (pI) 8.22

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5AO7 TRANSFERASE 09/09/15 Crystal Structure of SltB3 from Pseudomonas aeruginosa in complex with NAG-anhNAM-pentapeptide PSEUDOMONAS AERUGINOSA 2.09 X-RAY DIFFRACTION 100.0
5ANZ HYDROLASE 09/09/15 Crystal Structure of SltB3 from Pseudomonas aeruginosa. PSEUDOMONAS AERUGINOSA 1.614 X-RAY DIFFRACTION 100.0
5AO8 TRANSFERASE 09/09/15 Crystal Structure of SltB3 from Pseudomonas aeruginosa in complex with NAG-NAM-pentapeptide PSEUDOMONAS AERUGINOSA 2.23 X-RAY DIFFRACTION 99.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 226 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001689 (557 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3992
Search term: sltB3
Search term: SltB3

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_68_E2
Genomic Position 4472967
Position in ORF 837(1347)
Reference 15805499
Type ISlacZ/hah insertion site
Mutant ID UWGC: 16156
Genomic Position 4472681
Position in ORF 551(1347)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 21394
Genomic Position 4472544
Position in ORF 414(1347)
Reference 14617778

Transposon Insertions in Orthologs

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Characterization of soluble and membrane-bound family 3 lytic transglycosylases from Pseudomonas aeruginosa.
Blackburn NT, Clarke AJ
Biochemistry 2002 Jan;41(3):1001-13
PubMed ID: 11790124