Pseudomonas aeruginosa PAO1, PA4043 (ispA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4043
Name	ispA
Replicon	chromosome
Genomic location	4525551 - 4526438 (+ strand)
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_252732.1
GI	15599238
Affymetrix	PA4043_ispA_at
DNASU	PaCD00007766
Entrez	879058
GenBank	AAG07430.1
INSDC	AAG07430.1
NCBI Locus Tag	PA4043
protein_id(GenBank)	gb|AAG07430.1|AE004821_3|gnl|PseudoCAP|PA4043
TIGR	NTL03PA04043
UniParc	UPI00000C5BD9
UniProtKB Acc	Q9HWY4
UniProtKB ID	Q9HWY4_PSEAE
UniRef100	UniRef100_Q9HWY4
UniRef50	UniRef50_A0A011QRS6
UniRef90	UniRef90_Q9HWY4

Product

Feature Type	CDS
Coding Frame	1
Product Name	geranyltranstransferase Product Name Confidence: Class 2
Synonyms	farnesyldiphosphate synthase FPP synthase
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-8.43
Kyte-Doolittle Hydrophobicity Value	-0.046
Molecular Weight (kDa)	31.5
Isoelectric Point (pI)	5.06

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 2 Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
3ZOU	TRANSFERASE	02/25/13	Native structure of Farnesyl Pyrophosphate Synthase from Pseudomonas aeruginosa PA01, with bound fragment SPB02696, and substrate geranyl pyrophosphate.	PSEUDOMONAS AERUGINOSA PAO1	1.55	X-RAY DIFFRACTION	100.0
3ZL6	TRANSFERASE	01/28/13	Native structure of Farnesyl Pyrophosphate Synthase from Pseudomonas aeruginosa PAO1, with bound fragment KM10833.	PSEUDOMONAS AERUGINOSA PAO1	1.85	X-RAY DIFFRACTION	100.0
4UMJ	TRANSFERASE	05/18/14	Native structure of Farnesyl Pyrophosphate Synthase from Pseudomonas aeruginosa PA01, with bound ibandronic acid molecules.	PSEUDOMONAS AERUGINOSA	1.85	X-RAY DIFFRACTION	100.0
3ZMC	TRANSFERASE	02/07/13	Native structure of Farnesyl Pyrophosphate Synthase from Pseudomonas aeruginosa PA01, with bound substrate molecule Geranyl pyrophosphate.	PSEUDOMONAS AERUGINOSA PAO1	1.87	X-RAY DIFFRACTION	100.0
3ZMB	TRANSFERASE	02/07/13	Native structure of Farnesyl Pyrophosphate Synthase from Pseudomonas aeruginosa PA01, with bound fragment SPB02696.	PSEUDOMONAS AERUGINOSA PAO1	1.9	X-RAY DIFFRACTION	100.0
3ZCD	TRANSFERASE	11/19/12	Native structure of Farnesyl Pyrophosphate Synthase from Pseudomonas aeruginosa PA01.	PSEUDOMONAS AERUGINOSA PAO1	1.55	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 656 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001641 (538 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4043 Search term: ispA Search term: geranyltranstransferase

Human Homologs

References

Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli. Fujisaki S, Hara H, Nishimura Y, Horiuchi K, Nishino T J Biochem 1990 Dec;108(6):995-1000 PubMed ID: 2089044

The complete genome sequence of Escherichia coli K-12. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y Science 1997 Sep 5;277(5331):1453-62 PubMed ID: 9278503

Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis. Lois LM, Campos N, Putra SR, Danielsen K, Rohmer M, Boronat A Proc Natl Acad Sci U S A 1998 Mar 3;95(5):2105-10 PubMed ID: 9482846