Pseudomonas aeruginosa PAO1, PA4175 (piv)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4175
Name	piv Synonym: prpL Synonym: piv
Replicon	chromosome
Genomic location	4671319 - 4672707 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	protease IV is an ocular virulence factor (rabbit model).

Cross-References

RefSeq	NP_252864.1
GI	15599370
Affymetrix	PA4175_at
Entrez	880208
GenBank	AAG07562.1
INSDC	AAG07562.1
NCBI Locus Tag	PA4175
protein_id(GenBank)	gb|AAG07562.1|AE004834_2|gnl|PseudoCAP|PA4175
TIGR	NTL03PA04175
UniParc	UPI00000C5C54
UniProtKB Acc	Q9HWK6
UniProtKB ID	LYSC_PSEAE
UniRef100	UniRef100_Q9HWK6
UniRef50	UniRef50_Q9HWK6
UniRef90	UniRef90_Q9HWK6

Product

Feature Type	CDS
Coding Frame	1
Product Name	protease IV Product Name Confidence: Class 1
Synonyms	Pvds-regulated endoprotease, lysyl class protease IV probable endoproteinase Arg-C precursor
Evidence for Translation
Charge (pH 7)	-0.34
Kyte-Doolittle Hydrophobicity Value	-0.097
Molecular Weight (kDa)	48.2
Isoelectric Point (pI)	6.94

Subcellular localization

Individual Mappings	Localization Confidence PMID Extracellular Class 3 20472543 Extracellular Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database	VFDB
Category	Protease IV
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 24 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001524 (296 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4175 Search term: piv Search term: protease IV

Human Homologs

References

Characterization of an endoprotease (PrpL) encoded by a PvdS-regulated gene in Pseudomonas aeruginosa. Wilderman PJ, Vasil AI, Johnson Z, Wilson MJ, Cunliffe HE, Lamont IL, Vasil ML Infect Immun 2001 Sep;69(9):5385-94 PubMed ID: 11500408

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Cloning of a Lysobacter enzymogenes gene that encodes an arginyl endopeptidase (endoproteinase Arg-C). Wright DS, Graham LD, Jennings PA Biochim Biophys Acta 1998 Dec 22;1443(3):369-74 PubMed ID: 9878833

Pseudomonas keratitis. The role of an uncharacterized exoprotein, protease IV, in corneal virulence. O'Callaghan RJ, Engel LS, Hobden JA, Callegan MC, Green LC, Hill JM Invest Ophthalmol Vis Sci 1996 Mar;37(4):534-43 PubMed ID: 8595953

Pseudomonas deficient in protease IV has significantly reduced corneal virulence. Engel LS, Hobden JA, Moreau JM, Callegan MC, Hill JM, O'Callaghan RJ Invest Ophthalmol Vis Sci 1997 Jul;38(8):1535-42 PubMed ID: 9224281

Pseudomonas aeruginosa protease IV produces corneal damage and contributes to bacterial virulence. Engel LS, Hill JM, Moreau JM, Green LC, Hobden JA, O'Callaghan RJ Invest Ophthalmol Vis Sci 1998 Mar;39(3):662-5 PubMed ID: 9501882

Protease IV, a unique extracellular protease and virulence factor from Pseudomonas aeruginosa. Engel LS, Hill JM, Caballero AR, Green LC, O'Callaghan RJ J Biol Chem 1998 Jul 3;273(27):16792-7 PubMed ID: 9642237

Pseudomonas aeruginosa protease IV enzyme assays and comparison to other Pseudomonas proteases. Caballero AR, Moreau JM, Engel LS, Marquart ME, Hill JM, O'Callaghan RJ Anal Biochem 2001 Mar;290(2):330-7 PubMed ID: 11237336

Identification of the active site residues of Pseudomonas aeruginosa protease IV. Importance of enzyme activity in autoprocessing and activation. Traidej M, Marquart ME, Caballero AR, Thibodeaux BA, O'Callaghan RJ J Biol Chem 2003 Jan 24;278(4):2549-53 PubMed ID: 12419815

Pseudomonas keratitis: protease IV gene conservation, distribution, and production relative to virulence and other Pseudomonas proteases. Caballero A, Thibodeaux B, Marquart M, Traidej M, O'Callaghan R Invest Ophthalmol Vis Sci 2004 Feb;45(2):522-30 PubMed ID: 14744894

Molecular analysis of Pseudomonas aeruginosa protease IV expressed in Pseudomonas putida. Traidej M, Caballero AR, Marquart ME, Thibodeaux BA, O'Callaghan RJ Invest Ophthalmol Vis Sci 2003 Jan;44(1):190-6 PubMed ID: 12506074

Calcium and magnesium enhance the production of Pseudomonas aeruginosa protease IV, a corneal virulence factor. Marquart ME, Dajcs JJ, Caballero AR, Thibodeaux BA, O'Callaghan RJ Med Microbiol Immunol 2005 Jan;194(1-2):39-45 PubMed ID: 14628143

Pseudomonas aeruginosa protease IV: a corneal virulence factor of low immunogenicity. Thibodeaux BA, Caballero AR, Dajcs JJ, Marquart ME, Engel LS, O'Callaghan RJ Ocul Immunol Inflamm 2005 Apr-Jun;13(2-3):169-82 PubMed ID: 16019676

Pseudomonas aeruginosa protease IV degrades surfactant proteins and inhibits surfactant host defense and biophysical functions. Malloy JL, Veldhuizen RA, Thibodeaux BA, O'Callaghan RJ, Wright JR Am J Physiol Lung Cell Mol Physiol 2005 Feb;288(2):L409-18 PubMed ID: 15516485

Emergence of secretion-defective sublines of Pseudomonas aeruginosa PAO1 resulting from spontaneous mutations in the vfr global regulatory gene. Fox A, Haas D, Reimmann C, Heeb S, Filloux A, Voulhoux R Appl Environ Microbiol 2008 Mar;74(6):1902-8 PubMed ID: 18203852

Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa. Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M J Bacteriol 2015 Feb 15;197(4):762-73 PubMed ID: 25488299