Pseudomonas aeruginosa PAO1, PA4178 (eftM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4178
Name
eftM
Replicon chromosome
Genomic location 4673963 - 4674706 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_252867.1
GI 15599373
Affymetrix PA4178_at
Entrez 880256
GenBank AAG07565.1
INSDC AAG07565.1
NCBI Locus Tag PA4178
protein_id(GenBank) gb|AAG07565.1|AE004834_5|gnl|PseudoCAP|PA4178
TIGR NTL03PA04178
UniParc UPI00000C5C57
UniProtKB Acc Q9HWK3
UniProtKB ID Q9HWK3_PSEAE
UniRef100 UniRef100_Q9HWK3
UniRef50 UniRef50_Q5E5R1
UniRef90 UniRef90_W0YY28

Product

Feature Type CDS
Coding Frame 1
Product Name
SAM-dependent methyltransferase , EftM
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -4.64
Kyte-Doolittle Hydrophobicity Value -0.355
Molecular Weight (kDa) 28.1
Isoelectric Point (pI) 6.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 139 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001521 (354 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4178
Search term: eftM

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 21901
Genomic Position 4674168
Position in ORF 206(744)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 3535
Genomic Position 4674225
Position in ORF 263(744)
Reference 14617778

Transposon Insertions in Orthologs

References

Hybrid anthracycline antibiotics: production of new anthracyclines by cloned genes from Streptomyces purpurascens in Streptomyces galilaeus.
Niemi J, Ylihonko K, Hakala J, Pärssinen R, Kopio A, Mäntsälä P
Microbiology (Reading, Engl.) 1994 Jun;140 ( Pt 6)
PubMed ID: 8081500
Pseudomonas aeruginosa EftM Is a Thermoregulated Methyltransferase.
Owings JP, Kuiper EG, Prezioso SM, Meisner J, Varga JJ, Zelinskaya N, Dammer EB, Duong DM, Seyfried NT, Albertí S, Conn GL, Goldberg JB
J. Biol. Chem. 2016 Feb;291(7):3280-90
PubMed ID: 26677219
Lysine trimethylation of EF-Tu mimics platelet-activating factor to initiate Pseudomonas aeruginosa pneumonia.
Barbier M, Owings JP, Martínez-Ramos I, Damron FH, Gomila R, Blázquez J, Goldberg JB, Albertí S
MBio 2013 May;4(3):e00207-13
PubMed ID: 23653444