Pseudomonas aeruginosa PAO1, PA4197 (bfiS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4197
Name
bfiS
Replicon chromosome
Genomic location 4693759 - 4696035 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_252886.1
GI 15599392
Affymetrix PA4197_at
Entrez 880350
GenBank AAG07584.1
INSDC AAG07584.1
NCBI Locus Tag PA4197
protein_id(GenBank) gb|AAG07584.1|AE004836_3|gnl|PseudoCAP|PA4197
TIGR NTL03PA04197
UniParc UPI00000C5C6A
UniProtKB Acc Q9HWI4
UniProtKB ID Q9HWI4_PSEAE
UniRef100 UniRef100_Q9HWI4
UniRef50 UniRef50_Q9HWI4
UniRef90 UniRef90_Q9HWI4

Product

Feature Type CDS
Coding Frame 1
Product Name
BfiS
Product Name Confidence: Class 1
Synonyms Biofilm initiation Sensor
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.00
Kyte-Doolittle Hydrophobicity Value -0.253
Molecular Weight (kDa) 82.9
Isoelectric Point (pI) 6.88

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Lactuca sativa
Notes
Infection model Lettuce Infection model
Reference 20656909

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 597 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001509 (868 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4197
Search term: bfiS
Search term: BfiS

Human Homologs

References

The regulatory status of the fixL- and fixJ-like genes in Bradyrhizobium japonicum may be different from that in Rhizobium meliloti.
Anthamatten D, Hennecke H
Mol Gen Genet 1991 Jan;225(1):38-48
PubMed ID: 2000090
A novel signaling network essential for regulating Pseudomonas aeruginosa biofilm development.
Petrova OE, Sauer K
PLoS Pathog 2009 Nov;5(11):e1000668
PubMed ID: 19936057
The novel two-component regulatory system BfiSR regulates biofilm development by controlling the small RNA rsmZ through CafA.
Petrova OE, Sauer K
J Bacteriol 2010 Oct;192(20):5275-88
PubMed ID: 20656909