Pseudomonas aeruginosa PAO1, PA4202 (nmoA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4202
Name
nmoA
Replicon chromosome
Genomic location 4702042 - 4703097 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252891.1
GI 15599397
Affymetrix PA4202_at
Entrez 879034
GenBank AAG07589.1
INSDC AAG07589.1
NCBI Locus Tag PA4202
protein_id(GenBank) gb|AAG07589.1|AE004836_8|gnl|PseudoCAP|PA4202
TIGR NTL03PA04202
UniParc UPI00000C5C6F
UniProtKB Acc Q9HWH9
UniProtKB ID Q9HWH9_PSEAE
UniRef100 UniRef100_Q9HWH9
UniRef50 UniRef50_Q9HWH9
UniRef90 UniRef90_Q9HWH9

Product

Feature Type CDS
Coding Frame 1
Product Name
nitronate monooxygenase NmoA
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.88
Kyte-Doolittle Hydrophobicity Value 0.144
Molecular Weight (kDa) 36.8
Isoelectric Point (pI) 8.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4Q4K OXIDOREDUCTASE 04/14/14 Crystal structure of nitronate monooxygenase from Pseudomonas aeruginosa PAO1 PSEUDOMONAS AERUGINOSA 1.44 X-RAY DIFFRACTION 100.0
4QIS OXIDOREDUCTASE 06/02/14 Crystal structure of Nitroalkane Oxidase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.906 X-RAY DIFFRACTION 100.0
4QIT OXIDOREDUCTASE 06/02/14 Crystal structure of Nitroalkane Oxidase from Pseudomonas aeruginosa in mutant complex form PSEUDOMONAS AERUGINOSA 1.4 X-RAY DIFFRACTION 99.7
4QIU OXIDOREDUCTASE 06/02/14 Crystal structure of Nitroalkane Oxidase from Pseudomonas aeruginosa in Mutant complex form PSEUDOMONAS AERUGINOSA 1.401 X-RAY DIFFRACTION 99.7

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 361 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001505 (495 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4202
Search term: nmoA

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 37318
Genomic Position 4702583
Position in ORF 542(1056)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 44421
Genomic Position 4702832
Position in ORF 791(1056)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_09580
Mutant ID PA14NR: 32302
Genomic Position 824085
Position in ORF 253(1056)
Reference 16477005

References

Unique primary structure of 2-nitropropane dioxygenase from Hansenula mrakii.
Tchorzewski M, Kurihara T, Esaki N, Soda K.
Eur J Biochem. 1994 Dec 15;226(3):841-6.
PubMed ID: 7813473
The combined structural and kinetic characterization of a bacterial nitronate monooxygenase from Pseudomonas aeruginosa PAO1 establishes NMO class I and II.
Salvi F(1), Agniswamy J(2), Yuan H(1), Vercammen K(3), Pelicaen R(3), Cornelis P(3), Spain JC(4), Weber IT(5), Gadda G(6).
J Biol Chem. 2014 Aug 22;289(34):23764-75.
PubMed ID: 25002579
PubMed ID: 25384477