Pseudomonas aeruginosa PAO1, PA4332 (sadC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4332
Name
sadC
Replicon chromosome
Genomic location 4860248 - 4861711 (- strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_253022
GI 15599528
Affymetrix PA4332_at
Entrez 881493
GenBank AAG07720.1
NCBI Locus Tag PA4332
protein_id(GenBank) gb|AAG07720.1|AE004849_7|gnl|PseudoCAP|PA4332
TIGR NTL03PA04333
UniProtKB Acc Q9HW69
UniProtKB ID Q9HW69_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
SadC
Product Name Confidence: Class 1
CommentOverexpression of PA4332 causes increased pellicle formation
Evidence for Translation
Charge (pH 7) 18.37
Kyte-Doolittle Hydrophobicity Value -0.030
Molecular Weight (kDa) 54.1
Isoelectric Point (pI) 9.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 1
green fluorescent protein fusion protein localization evidence
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4WXW TRANSFERASE 11/14/14 SadC (323-487) from Pseudomonas aeruginosa PAO1 PSEUDOMONAS AERUGINOSA PAO1 1.8 X-RAY DIFFRACTION 100.0
4WXO TRANSFERASE 11/14/14 SadC (300-487) from Pseudomonas aeruginosa PAO1 PSEUDOMONAS AERUGINOSA PAO1 2.805 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 453 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000167 (2847 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4332
Search term: sadC
Search term: SadC

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 19370
Genomic Position 4860639
Position in ORF 392(1464)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 32422
Genomic Position 4861065
Position in ORF 818(1464)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_17_G10
Genomic Position 4860819
Position in ORF 572(1464)
Reference 15805499

Transposon Insertions in Orthologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
The diguanylate cyclase SadC is a central player in Gac/Rsm-mediated biofilm formation in Pseudomonas aeruginosa.
Moscoso JA, Jaeger T, Valentini M, Hui K, Jenal U, Filloux A
J. Bacteriol. 2014 Dec;196(23):4081-8
PubMed ID: 25225264
Specific control of Pseudomonas aeruginosa surface-associated behaviors by two c-di-GMP diguanylate cyclases.
Merritt JH, Ha DG, Cowles KN, Lu W, Morales DK, Rabinowitz J, Gitai Z, O'Toole GA
MBio 2010 Oct;1(4):
PubMed ID: 20978535
SadC reciprocally influences biofilm formation and swarming motility via modulation of exopolysaccharide production and flagellar function.
Merritt JH, Brothers KM, Kuchma SL, O'Toole GA
J. Bacteriol. 2007 Nov;189(22):8154-64
PubMed ID: 17586642
Synergistic interaction of glyceraldehydes-3-phosphate dehydrogenase and ArsJ, a novel organoarsenical efflux permease, confers arsenate resistance.
Chen J, Yoshinaga M, Garbinski LD, Rosen BP
Mol. Microbiol. 2016 Jun;100(6):945-53
PubMed ID: 26991003