Pseudomonas aeruginosa PAO1, PA4365 (lysE)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: yggA
Replicon chromosome
Genomic location 4892748 - 4893350 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_253055.1
GI 15599561
Affymetrix PA4365_at
DNASU PaCD00007890
Entrez 881347
GenBank AAG07753.1
INSDC AAG07753.1
NCBI Locus Tag PA4365
protein_id(GenBank) gb|AAG07753.1|AE004852_6|gnl|PseudoCAP|PA4365
UniParc UPI00000C5CE3
UniProtKB Acc Q9HW36
UniRef100 UniRef100_W0YXB1
UniRef50 UniRef50_M1WX56
UniRef90 UniRef90_W0YXB1


Feature Type CDS
Coding Frame 1
Product Name
Lysine efflux permease
Product Name Confidence: Class 3
Evidence for Translation
Charge (pH 7) 6.64
Kyte-Doolittle Hydrophobicity Value 0.910
Molecular Weight (kDa) 21.1
Isoelectric Point (pI) 10.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 184 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003958 (573 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA4365
Search term: lysE

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 19604
Genomic Position 4893345
Position in ORF 598(603)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 46832
Genomic Position 4892928
Position in ORF 181(603)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_56770
Mutant ID PA14NR: 33832
Genomic Position 5056314
Position in ORF 15(603)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_56770
Mutant ID PA14NR: 45000
Genomic Position 5056676
Position in ORF 377(603)
Reference 16477005


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PubMed ID: 15687295
Molecular characterization of lysR-lysXE, gcdR-gcdHG and amaR-amaAB operons for lysine export and catabolism: a comprehensive lysine catabolic network in Pseudomonas aeruginosa PAO1.
Madhuri Indurthi S(1), Chou HT(1), Lu CD(2).
Microbiology. 2016 May;162(5):876-88.
PubMed ID: 26967762