Pseudomonas aeruginosa PAO1, PA4367 (bifA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4367
Name
bifA
Synonym: bifA
Replicon chromosome
Genomic location 4894459 - 4896522 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentOverexpression of PA4367 impairs TTSS-mediated cytotoxicity

Cross-References

RefSeq NP_253057.1
GI 15599563
Affymetrix PA4367_at
Entrez 881428
GenBank AAG07755.1
INSDC AAG07755.1
NCBI Locus Tag PA4367
protein_id(GenBank) gb|AAG07755.1|AE004852_8|gnl|PseudoCAP|PA4367
TIGR NTL03PA04368
UniParc UPI00000C5CE4
UniProtKB Acc Q9HW35
UniProtKB ID Q9HW35_PSEAE
UniRef100 UniRef100_W0YZ94
UniRef50 UniRef50_D7I4A9
UniRef90 UniRef90_A0A0A8RHN1

Product

Feature Type CDS
Coding Frame 1
Product Name
BifA
Product Name Confidence: Class 1
Synonyms BifA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.51
Kyte-Doolittle Hydrophobicity Value -0.205
Molecular Weight (kDa) 77.5
Isoelectric Point (pI) 5.93

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
8ARV HYDROLASE 08/17/22 Structure of the EAL domain of BifA from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 1.9 X-RAY DIFFRACTION 98.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 449 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003960 (524 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4367
Search term: bifA
Search term: BifA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
BifA, a cyclic-Di-GMP phosphodiesterase, inversely regulates biofilm formation and swarming motility by Pseudomonas aeruginosa PA14.
Kuchma SL, Brothers KM, Merritt JH, Liberati NT, Ausubel FM, O'Toole GA
J Bacteriol 2007 Nov;189(22):8165-78
PubMed ID: 17586641