Pseudomonas aeruginosa PAO1, PA4370 (icmP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4370
Name
icmP
Replicon chromosome
Genomic location 4898193 - 4899533 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_253060.1
GI 15599566
Affymetrix PA4370_at
Entrez 881386
GenBank AAG07758.1
INSDC AAG07758.1
NCBI Locus Tag PA4370
protein_id(GenBank) gb|AAG07758.1|AE004853_1|gnl|PseudoCAP|PA4370
TIGR NTL03PA04371
UniParc UPI00000C5CE7
UniProtKB Acc Q9HW32
UniProtKB ID Q9HW32_PSEAE
UniRef100 UniRef100_Q9HW32
UniRef50 UniRef50_Q9HW32
UniRef90 UniRef90_Q9HW32

Product

Feature Type CDS
Coding Frame 1
Product Name
Insulin-cleaving metalloproteinase outer membrane protein precursor
Product Name Confidence: Class 1
Synonyms outer membrane metalloproteinase precursor
insulin-cleaving membrane proteinase ICMP
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (47.23 kDa, PMID:21751344)
Charge (pH 7) -24.17
Kyte-Doolittle Hydrophobicity Value -0.291
Molecular Weight (kDa) 47.2
Isoelectric Point (pI) 4.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Vesicle Class 1
Outer Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7XRX METAL BINDING PROTEIN 05/12/22 insulin-cleaving membrane protease-ICMP Pseudomonas aeruginosa PAO1 1.882 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 70 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003962 (528 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4370
Search term: icmP

Human Homologs

References

Characterization and purification of an outer membrane metalloproteinase from Pseudomonas aeruginosa with fibrinogenolytic activity.
Fricke B, Parchmann O, Kruse K, Rücknagel P, Schierhorn A, Menge S
Biochim Biophys Acta 1999 Aug 30;1454(3):236-50
PubMed ID: 10452958
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344