Pseudomonas aeruginosa PAO1, PA4374 (mexV)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4374
Name
mexV
Replicon chromosome
Genomic location 4903466 - 4904596 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253064.1
GI 15599570
Affymetrix PA4374_at
Entrez 881374
GenBank AAG07762.1
INSDC AAG07762.1
NCBI Locus Tag PA4374
protein_id(GenBank) gb|AAG07762.1|AE004853_5|gnl|PseudoCAP|PA4374
TIGR NTL03PA04375
UniParc UPI00000C5CEB
UniProtKB Acc Q9HW28
UniProtKB ID Q9HW28_PSEAE
UniRef100 UniRef100_X6YM01
UniRef50 UniRef50_U5VAD4
UniRef90 UniRef90_W0YYN3

Product

Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) multidrug efflux membrane fusion protein MexV
Product Name Confidence: Class 1
Synonyms probable RND efflux membrane fusion protein precursor
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (40.87 kDa, PMID:21751344)
Charge (pH 7) -3.50
Kyte-Doolittle Hydrophobicity Value -0.019
Molecular Weight (kDa) 40.9
Isoelectric Point (pI) 5.35

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
MexV MexV is the membrane fusion protein of the MexVW-OprM multidrug efflux complex. [PMID:12951344] ARO:3003030 protein homolog model Perfect 700.3 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 449 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001501 (811 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4374
Search term: mexV

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
A new member of the tripartite multidrug efflux pumps, MexVW-OprM, in Pseudomonas aeruginosa.
Li Y, Mima T, Komori Y, Morita Y, Kuroda T, Mizushima T, Tsuchiya T
J Antimicrob Chemother 2003 Oct;52(4):572-5
PubMed ID: 12951344