Pseudomonas aeruginosa PAO1, PA4375 (mexW)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4375
Name
mexW
Replicon chromosome
Genomic location 4904647 - 4907703 (+ strand)
Transposon Mutants 8 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_253065.1
GI 15599571
Affymetrix PA4375_at
Entrez 881377
GenBank AAG07763.1
INSDC AAG07763.1
NCBI Locus Tag PA4375
protein_id(GenBank) gb|AAG07763.1|AE004853_6|gnl|PseudoCAP|PA4375
TIGR NTL03PA04376
UniParc UPI00000C5CEC
UniProtKB Acc Q9HW27
UniProtKB ID Q9HW27_PSEAE
UniRef100 UniRef100_W0YXB9
UniRef50 UniRef50_G8Q6V3
UniRef90 UniRef90_W0YXB9

Product

Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) multidrug efflux transporter MexW
Product Name Confidence: Class 1
Synonyms probable RND efflux transporter
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -17.64
Kyte-Doolittle Hydrophobicity Value 0.361
Molecular Weight (kDa) 110.5
Isoelectric Point (pI) 4.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
MexW MexW is the RND-type membrane protein of the efflux complex MexVW-OprM. [PMID:12951344] ARO:3003031 protein homolog model Perfect 1906.3 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 487 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001500 (799 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4375
Search term: mexW

Human Homologs

References

A new member of the tripartite multidrug efflux pumps, MexVW-OprM, in Pseudomonas aeruginosa.
Li Y, Mima T, Komori Y, Morita Y, Kuroda T, Mizushima T, Tsuchiya T
J Antimicrob Chemother 2003 Oct;52(4):572-5
PubMed ID: 12951344