Pseudomonas aeruginosa PAO1, PA4398

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4398
Name
Replicon chromosome
Genomic location 4927998 - 4930094 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253088.1
GI 15599594
Affymetrix PA4398_at
Entrez 881355
GenBank AAG07786.1
INSDC AAG07786.1
NCBI Locus Tag PA4398
protein_id(GenBank) gb|AAG07786.1|AE004855_8|gnl|PseudoCAP|PA4398
TIGR NTL03PA04399
UniParc UPI00000C5CFC
UniProtKB Acc Q9HW08
UniProtKB ID Q9HW08_PSEAE
UniRef100 UniRef100_W0YYQ5
UniRef50 UniRef50_Q4K7C5
UniRef90 UniRef90_Q02H42

Product

Feature Type CDS
Coding Frame 1
Product Name
two-component sensor
Product Name Confidence: Class 1
Synonyms probable two-component sensor
histidine kinase protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.88
Kyte-Doolittle Hydrophobicity Value -0.208
Molecular Weight (kDa) 77.6
Isoelectric Point (pI) 5.71

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 580 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003988 (532 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4398

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.
Itoh T, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Kasai H, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Seki Y, Horiuchi T
DNA Res 1996 Dec 31;3(6):379-92
PubMed ID: 9097040
Swarming of Pseudomonas aeruginosa is controlled by a broad spectrum of transcriptional regulators, including MetR.
Yeung AT, Torfs EC, Jamshidi F, Bains M, Wiegand I, Hancock RE, Overhage J
J Bacteriol 2009 Sep;191(18):5592-602
PubMed ID: 19592586
Sensor kinase PA4398 modulates swarming motility and biofilm formation in Pseudomonas aeruginosa PA14.
Strehmel J, Neidig A, Nusser M, Geffers R, Brenner-Weiss G, Overhage J
Appl Environ Microbiol 2015 Feb;81(4):1274-85
PubMed ID: 25501476