Pseudomonas aeruginosa PAO1, PA4455

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4455
Name
Synonym: yrbE
Replicon chromosome
Genomic location 4987285 - 4988082 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_253145.1
GI 15599651
Affymetrix PA4455_at
DNASU PaCD00007931
Entrez 881008
GenBank AAG07843.1
INSDC AAG07843.1
NCBI Locus Tag PA4455
protein_id(GenBank) gb|AAG07843.1|AE004859_12|gnl|PseudoCAP|PA4455
TIGR NTL03PA04456
UniParc UPI00000C5D1A
UniProtKB Acc Q9HVW2
UniProtKB ID Q9HVW2_PSEAE
UniRef100 UniRef100_A0A072ZEI7
UniRef50 UniRef50_F6FXV9
UniRef90 UniRef90_A0A072ZEI7

Product

Feature Type CDS
Coding Frame 1
Product Name
probable permease of ABC transporter
Product Name Confidence: Class 1
Evidence for Translation
Detected using LC-MS/MS (28.38 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.44
Kyte-Doolittle Hydrophobicity Value 0.762
Molecular Weight (kDa) 28.4
Isoelectric Point (pI) 7.50

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 406 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004044 (561 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4455

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 32930
Genomic Position 4987880
Position in ORF 596(798)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 19354
Genomic Position 4987465
Position in ORF 181(798)
Reference 14617778

Transposon Insertions in Orthologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
A Putative ABC Transporter Permease Is Necessary for Resistance to Acidified Nitrite and EDTA in Pseudomonas aeruginosa under Aerobic and Anaerobic Planktonic and Biofilm Conditions.
McDaniel C, Su S, Panmanee W, Lau GW, Browne T, Cox K, Paul AT, Ko SH, Mortensen JE, Lam JS, Muruve DA, Hassett DJ
Front Microbiol 2016 ;7
PubMed ID: 27064218