Pseudomonas aeruginosa PAO1, PA4496 (dppA1)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 5031488 - 5033101 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1


RefSeq NP_253186.1
GI 15599692
Affymetrix PA4496_at
Entrez 881067
GenBank AAG07884.1
INSDC AAG07884.1
NCBI Locus Tag PA4496
protein_id(GenBank) gb|AAG07884.1|AE004863_4|gnl|PseudoCAP|PA4496
UniParc UPI00000C5D38
UniProtKB Acc Q9HVS5
UniRef100 UniRef100_W0Z289
UniRef50 UniRef50_Q4KIC0
UniRef90 UniRef90_V6ALT5


Feature Type CDS
Coding Frame 1
Product Name
probable binding protein component of ABC transporter
Product Name Confidence: Class 1
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.06
Kyte-Doolittle Hydrophobicity Value -0.465
Molecular Weight (kDa) 60.1
Isoelectric Point (pI) 7.62

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Periplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 559 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004084 (840 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA4496
Search term: dppA1

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 30225
Genomic Position 5032774
Position in ORF 1287(1614)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 32242
Genomic Position 5032423
Position in ORF 936(1614)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_46_H6
Genomic Position 5032954
Position in ORF 1467(1614)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_21_E1
Genomic Position 5033013
Position in ORF 1526(1614)
Reference 15805499

Transposon Insertions in Orthologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
High-throughput screening of dipeptide utilization mediated by the ABC transporter DppBCDF and its substrate-binding proteins DppA1-A5 in Pseudomonas aeruginosa.
Pletzer D, Lafon C, Braun Y, Köhler T, Page MG, Mourez M, Weingart H
PLoS ONE 2014 ;9(10):e111311
PubMed ID: 25338022