Pseudomonas aeruginosa PAO1, PA4496 (dppA1)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4496
Name	dppA1
Replicon	chromosome
Genomic location	5031488 - 5033101 (+ strand)
Transposon Mutants	4 transposon mutants in PAO1
Transposon Mutants in orthologs	3 transposon mutants in orthologs
Comment	Structural similarity to 1DPE (dipeptide binding protein from Escherichia coli, root-mean-square deviation: 0.5 angstroms). Sequence similarity to 1DPP (99%/53,06%) (G-L). Final predicted ligand: dipeptide (PMID: 31631799)

Cross-References

RefSeq	NP_253186.1
GI	15599692
Affymetrix	PA4496_at
Entrez	881067
GenBank	AAG07884.1
INSDC	AAG07884.1
NCBI Locus Tag	PA4496
protein_id(GenBank)	gb|AAG07884.1|AE004863_4|gnl|PseudoCAP|PA4496
TIGR	NTL03PA04497
UniParc	UPI00000C5D38
UniProtKB Acc	Q9HVS5
UniProtKB ID	Q9HVS5_PSEAE
UniRef100	UniRef100_W0Z289
UniRef50	UniRef50_Q4KIC0
UniRef90	UniRef90_V6ALT5

Product

Feature Type	CDS
Coding Frame	1
Product Name	probable binding protein component of ABC transporter Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7)	2.06
Kyte-Doolittle Hydrophobicity Value	-0.465
Molecular Weight (kDa)	60.1
Isoelectric Point (pI)	7.62

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 2 Periplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 559 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004084 (810 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4496 Search term: dppA1 Search term: probable binding protein component of ABC transporter

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

High-throughput screening of dipeptide utilization mediated by the ABC transporter DppBCDF and its substrate-binding proteins DppA1-A5 in Pseudomonas aeruginosa. Pletzer D, Lafon C, Braun Y, Köhler T, Page MG, Mourez M, Weingart H PLoS One 2014;9(10):e111311 PubMed ID: 25338022

Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach. Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F J Biomol Struct Dyn 2020 Sep;38(15):4508-4520 PubMed ID: 31631799