Pseudomonas aeruginosa PAO1, PA4500 (dppA3)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 5037074 - 5038675 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1


RefSeq NP_253190.1
GI 15599696
Affymetrix PA4500_at
Entrez 881062
GenBank AAG07888.1
INSDC AAG07888.1
NCBI Locus Tag PA4500
protein_id(GenBank) gb|AAG07888.1|AE004863_8|gnl|PseudoCAP|PA4500
UniParc UPI00000C5D3C
UniProtKB Acc Q9HVS1
UniRef100 UniRef100_Q9HVS1
UniRef50 UniRef50_Q4KIC0
UniRef90 UniRef90_V6AL29


Feature Type CDS
Coding Frame 1
Product Name
probable binding protein component of ABC transporter
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 4.53
Kyte-Doolittle Hydrophobicity Value -0.430
Molecular Weight (kDa) 59.7
Isoelectric Point (pI) 8.26

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 560 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004084 (840 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA4500
Search term: dppA3

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 33179
Genomic Position 5037346
Position in ORF 273(1602)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 32399
Genomic Position 5037556
Position in ORF 483(1602)
Reference 14617778

Transposon Insertions in Orthologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
High-throughput screening of dipeptide utilization mediated by the ABC transporter DppBCDF and its substrate-binding proteins DppA1-A5 in Pseudomonas aeruginosa.
Pletzer D, Lafon C, Braun Y, Köhler T, Page MG, Mourez M, Weingart H
PLoS ONE 2014 ;9(10):e111311
PubMed ID: 25338022