Pseudomonas aeruginosa PAO1, PA4500 (dppA3)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4500
Name
dppA3
Replicon chromosome
Genomic location 5037074 - 5038675 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs
CommentStructural similarity to 1DPE (dipeptide binding protein from Escherichia coli, root-mean-square deviation: 0.4 angstroms). Sequence similarity to 1DPP (98%/53,16%) (G-L). Final predicted ligand: dipeptide (PMID: 31631799)

Cross-References

RefSeq NP_253190.1
GI 15599696
Affymetrix PA4500_at
Entrez 881062
GenBank AAG07888.1
INSDC AAG07888.1
NCBI Locus Tag PA4500
protein_id(GenBank) gb|AAG07888.1|AE004863_8|gnl|PseudoCAP|PA4500
TIGR NTL03PA04501
UniParc UPI00000C5D3C
UniProtKB Acc Q9HVS1
UniProtKB ID Q9HVS1_PSEAE
UniRef100 UniRef100_Q9HVS1
UniRef50 UniRef50_Q4KIC0
UniRef90 UniRef90_V6AL29

Product

Feature Type CDS
Coding Frame 1
Product Name
probable binding protein component of ABC transporter
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 4.53
Kyte-Doolittle Hydrophobicity Value -0.430
Molecular Weight (kDa) 59.7
Isoelectric Point (pI) 8.26

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 560 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004084 (810 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4500
Search term: dppA3

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
High-throughput screening of dipeptide utilization mediated by the ABC transporter DppBCDF and its substrate-binding proteins DppA1-A5 in Pseudomonas aeruginosa.
Pletzer D, Lafon C, Braun Y, Köhler T, Page MG, Mourez M, Weingart H
PLoS One 2014;9(10):e111311
PubMed ID: 25338022
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799