Pseudomonas aeruginosa PAO1, PA4502 (dppA4)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4502
Name
dppA4
Replicon chromosome
Genomic location 5040405 - 5042000 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253192.1
GI 15599698
Affymetrix PA4502_at
Entrez 881096
GenBank AAG07890.1
INSDC AAG07890.1
NCBI Locus Tag PA4502
protein_id(GenBank) gb|AAG07890.1|AE004864_2|gnl|PseudoCAP|PA4502
TIGR NTL03PA04503
UniParc UPI00000C5D3E
UniProtKB Acc Q9HVR9
UniProtKB ID Q9HVR9_PSEAE
UniRef100 UniRef100_Q9HVR9
UniRef50 UniRef50_Q87WI5
UniRef90 UniRef90_Q9HVR9

Product

Feature Type CDS
Coding Frame 1
Product Name
probable binding protein component of ABC transporter
Product Name Confidence: Class 1
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.49
Kyte-Doolittle Hydrophobicity Value -0.262
Molecular Weight (kDa) 58.6
Isoelectric Point (pI) 6.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 559 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004089 (529 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4502
Search term: dppA4

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_24_E1
Genomic Position 5040619
Position in ORF 215(1596)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_20_E1
Genomic Position 5040619
Position in ORF 215(1596)
Reference 15805499
Type ISphoA/hah insertion site
Mutant ID UWGC: 33118
Genomic Position 5041910
Position in ORF 1506(1596)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_20_C1
Genomic Position 5040621
Position in ORF 217(1596)
Reference 15805499
Type ISphoA/hah insertion site
Mutant ID UWGC: 55965
Genomic Position 5041248
Position in ORF 844(1596)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_58420
Mutant ID PA14NR: 44163
Genomic Position 5205538
Position in ORF 1552(1602)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_58420
Mutant ID PA14NR: 57090
Genomic Position 5204117
Position in ORF 131(1602)
Reference 16477005

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM.
Proteomics. 2009 Apr;9(7):1901-15.
PubMed ID: 19333994
High-throughput screening of dipeptide utilization mediated by the ABC transporter DppBCDF and its substrate-binding proteins DppA1-A5 in Pseudomonas aeruginosa.
Pletzer D(1), Lafon C(2), Braun Y(1), Köhler T(3), Page MG(4), Mourez M(2), Weingart H(1).
PLoS One. 2014 Oct 22;9(10):e111311.
PubMed ID: 25338022