Pseudomonas aeruginosa PAO1, PA4502 (dppA4)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4502
Name
dppA4
Replicon chromosome
Genomic location 5040405 - 5042000 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentStructural similarity to 1DPE (dipeptide binding protein from Escherichia coli, root-mean-square deviation: 0.5 angstroms). Sequence similarity to 1DPP (99%/50,20%) (G-L). Final predicted ligand: dipeptide (PMID: 31631799)

Cross-References

RefSeq NP_253192.1
GI 15599698
Affymetrix PA4502_at
Entrez 881096
GenBank AAG07890.1
INSDC AAG07890.1
NCBI Locus Tag PA4502
protein_id(GenBank) gb|AAG07890.1|AE004864_2|gnl|PseudoCAP|PA4502
TIGR NTL03PA04503
UniParc UPI00000C5D3E
UniProtKB Acc Q9HVR9
UniProtKB ID Q9HVR9_PSEAE
UniRef100 UniRef100_Q9HVR9
UniRef50 UniRef50_Q87WI5
UniRef90 UniRef90_Q9HVR9

Product

Feature Type CDS
Coding Frame 1
Product Name
probable binding protein component of ABC transporter
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -2.49
Kyte-Doolittle Hydrophobicity Value -0.262
Molecular Weight (kDa) 58.6
Isoelectric Point (pI) 6.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 559 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004089 (498 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4502
Search term: dppA4

Human Homologs

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
High-throughput screening of dipeptide utilization mediated by the ABC transporter DppBCDF and its substrate-binding proteins DppA1-A5 in Pseudomonas aeruginosa.
Pletzer D, Lafon C, Braun Y, Köhler T, Page MG, Mourez M, Weingart H
PLoS One 2014;9(10):e111311
PubMed ID: 25338022
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799