Pseudomonas aeruginosa PAO1, PA4506 (dppF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4506
Name
dppF
Replicon chromosome
Genomic location 5045028 - 5045999 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253196.1
GI 15599702
Affymetrix PA4506_at
Entrez 881159
GenBank AAG07894.1
INSDC AAG07894.1
NCBI Locus Tag PA4506
protein_id(GenBank) gb|AAG07894.1|AE004864_6|gnl|PseudoCAP|PA4506
TIGR NTL03PA04507
UniParc UPI00000C5D42
UniProtKB Acc Q9HVR5
UniProtKB ID Q9HVR5_PSEAE
UniRef100 UniRef100_W0YZ53
UniRef50 UniRef50_U4KA71
UniRef90 UniRef90_W0YZ53

Product

Feature Type CDS
Coding Frame 1
Product Name
dipeptide ABC transporter ATP-binding protein DppF
Product Name Confidence: Class 1
Evidence for Translation
Detected using LC-MS/MS (36.34 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 10.49
Kyte-Doolittle Hydrophobicity Value -0.416
Molecular Weight (kDa) 36.3
Isoelectric Point (pI) 9.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004093 (545 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4506
Search term: dppF

Human Homologs

Ensembl 86, assembly GRCh38.p7
ATP binding cassette subfamily B member 7 [Source:HGNC Symbol;Acc:HGNC:48]
E-value: 1.6e-22
Percent Identity: 33.8

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_41_D4
Genomic Position 5045746
Position in ORF 719(972)
Reference 15805499
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 18549
Genomic Position 5045582
Position in ORF 555(972)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 4963
Genomic Position 5045640
Position in ORF 613(972)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_58490
Mutant ID PA14NR: 25289
Genomic Position 5209361
Position in ORF 746(972)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_58490
Mutant ID PA14NR: 46062
Genomic Position 5208916
Position in ORF 301(972)
Reference 16477005

References

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS.
Proteomics. 2011 Aug;11(16):3424-9.
PubMed ID: 21751344
High-throughput screening of dipeptide utilization mediated by the ABC transporter DppBCDF and its substrate-binding proteins DppA1-A5 in Pseudomonas aeruginosa.
Pletzer D(1), Lafon C(2), Braun Y(1), Köhler T(3), Page MG(4), Mourez M(2), Weingart H(1).
PLoS One. 2014 Oct 22;9(10):e111311.
PubMed ID: 25338022