Pseudomonas aeruginosa PAO1, PA4541 (lepA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4541
Name
lepA
Synonym: tpsA4
Replicon chromosome
Genomic location 5082443 - 5086696 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253231.1
GI 15599737
Affymetrix PA4541_at
Entrez 878626
GenBank AAG07929.1
INSDC AAG07929.1
NCBI Locus Tag PA4541
protein_id(GenBank) gb|AAG07929.1|AE004867_15|gnl|PseudoCAP|PA4541
TIGR NTL03PA04542
UniParc UPI00000C5D5C
UniProtKB Acc Q9HVN6
UniProtKB ID Q9HVN6_PSEAE
UniRef100 UniRef100_Q9HVN6
UniRef50 UniRef50_Q9HVN6
UniRef90 UniRef90_Q9HVN6

Product

Feature Type CDS
Coding Frame 1
Product Name
Pseudomonas aeruginosa-derived large extracellular protease, LepA
Product Name Confidence: Class 1
Synonyms LepA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 7.98
Kyte-Doolittle Hydrophobicity Value -0.045
Molecular Weight (kDa) 140.0
Isoelectric Point (pI) 8.56

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category Pseudomonas aeruginosa-derived large extracellular protease, LepA
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse model of acute systemic infection
Reference 21041488

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 134 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001605 (334 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4541
Search term: lepA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning, expression, and DNA sequence analysis of genes encoding nontypeable Haemophilus influenzae high-molecular-weight surface-exposed proteins related to filamentous hemagglutinin of Bordetella pertussis.
Barenkamp SJ, Leininger E
Infect Immun 1992 Apr;60(4):1302-13
PubMed ID: 1548058
A novel secreted protease from Pseudomonas aeruginosa activates NF-kappaB through protease-activated receptors.
Kida Y, Higashimoto Y, Inoue H, Shimizu T, Kuwano K
Cell Microbiol 2008 Jul;10(7):1491-504
PubMed ID: 18331590
Cooperation between LepA and PlcH contributes to the in vivo virulence and growth of Pseudomonas aeruginosa in mice.
Kida Y, Shimizu T, Kuwano K
Infect Immun 2011 Jan;79(1):211-9
PubMed ID: 21041488
Structure-function analysis reveals that the Pseudomonas aeruginosa Tps4 two-partner secretion system is involved in CupB5 translocation.
Garnett JA, Muhl D, Douse CH, Hui K, Busch A, Omisore A, Yang Y, Simpson P, Marchant J, Waksman G, Matthews S, Filloux A
Protein Sci 2015 May;24(5):670-87
PubMed ID: 25641651