Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4541
|
Name |
lepA
Synonym: tpsA4 |
Replicon | chromosome |
Genomic location | 5082443 - 5086696 (+ strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_253231.1 |
GI | 15599737 |
Affymetrix | PA4541_at |
Entrez | 878626 |
GenBank | AAG07929.1 |
INSDC | AAG07929.1 |
NCBI Locus Tag | PA4541 |
protein_id(GenBank) | gb|AAG07929.1|AE004867_15|gnl|PseudoCAP|PA4541 |
TIGR | NTL03PA04542 |
UniParc | UPI00000C5D5C |
UniProtKB Acc | Q9HVN6 |
UniProtKB ID | Q9HVN6_PSEAE |
UniRef100 | UniRef100_Q9HVN6 |
UniRef50 | UniRef50_Q9HVN6 |
UniRef90 | UniRef90_Q9HVN6 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
Pseudomonas aeruginosa-derived large extracellular protease, LepA
|
Synonyms |
LepA |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 7.98 |
Kyte-Doolittle Hydrophobicity Value | -0.045 |
Molecular Weight (kDa) | 140.0 |
Isoelectric Point (pI) | 8.56 |
Individual Mappings | |
Additional evidence for subcellular localization |
Source 1 | |
Database | PseudoCAP |
Category | Pseudomonas aeruginosa-derived large extracellular protease, LepA |
Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
Host Organism | Mus musculus |
Notes | |
Infection model | Mouse model of acute systemic infection |
Reference | 21041488 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 134 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001605 (334 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4541
Search term: lepA
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Cloning, expression, and DNA sequence analysis of genes encoding nontypeable Haemophilus influenzae high-molecular-weight surface-exposed proteins related to filamentous hemagglutinin of Bordetella pertussis.
Barenkamp SJ, Leininger E
Infect Immun 1992 Apr;60(4):1302-13
PubMed ID: 1548058
|
A novel secreted protease from Pseudomonas aeruginosa activates NF-kappaB through protease-activated receptors.
Kida Y, Higashimoto Y, Inoue H, Shimizu T, Kuwano K
Cell Microbiol 2008 Jul;10(7):1491-504
PubMed ID: 18331590
|
Cooperation between LepA and PlcH contributes to the in vivo virulence and growth of Pseudomonas aeruginosa in mice.
Kida Y, Shimizu T, Kuwano K
Infect Immun 2011 Jan;79(1):211-9
PubMed ID: 21041488
|
Structure-function analysis reveals that the Pseudomonas aeruginosa Tps4 two-partner secretion system is involved in CupB5 translocation.
Garnett JA, Muhl D, Douse CH, Hui K, Busch A, Omisore A, Yang Y, Simpson P, Marchant J, Waksman G, Matthews S, Filloux A
Protein Sci 2015 May;24(5):670-87
PubMed ID: 25641651
|