Pseudomonas aeruginosa PAO1, PA4550 (fimU)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4550
Name
fimU
Replicon chromosome
Genomic location 5098212 - 5098718 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentAlgR positively regulates the fimU-pilVWXY1Y2E operon

Cross-References

RefSeq NP_253240.1
GI 15599746
Affymetrix PA4550_fimU_at
DNASU PaCD00007970
Entrez 879606
GenBank AAG07938.1
INSDC AAG07938.1
NCBI Locus Tag PA4550
protein_id(GenBank) gb|AAG07938.1|AE004869_2|gnl|PseudoCAP|PA4550
TIGR NTL03PA04551
UniParc UPI00000D420A
UniProtKB Acc G3XCZ0
UniProtKB ID G3XCZ0_PSEAE
UniRef100 UniRef100_W0YWT8
UniRef50 UniRef50_A6VBT7
UniRef90 UniRef90_W0YWT8

Product

Feature Type CDS
Coding Frame 1
Product Name
type 4 fimbrial biogenesis protein FimU
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 5.41
Kyte-Doolittle Hydrophobicity Value -0.065
Molecular Weight (kDa) 18.1
Isoelectric Point (pI) 9.88

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4IPV CELL ADHESION 01/10/13 Crystal structure of Pseudomonas aeruginosa (strain: PAO1) type IV minor pilin FimU in space group P65 Pseudomonas aeruginosa 1.4 X-RAY DIFFRACTION 100.0
4IPU CELL ADHESION 01/10/13 Crystal structure of Pseudomonas aeruginosa (strain: PAO1) type IV minor pilin FimU in space group P21 Pseudomonas aeruginosa 1.351 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IVa pili
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 102 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004211 (706 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4550
Search term: fimU

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Pseudomonas aeruginosa AlgR regulates type IV pilus biosynthesis by activating transcription of the fimU-pilVWXY1Y2E operon.
Belete B, Lu H, Wozniak DJ
J Bacteriol 2008 Mar;190(6):2023-30
PubMed ID: 18178737