Pseudomonas aeruginosa PAO1, PA4554 (pilY1)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4554
Name	pilY1
Replicon	chromosome
Genomic location	5100683 - 5104168 (+ strand)
Transposon Mutants	11 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	AlgR positively regulates the fimU-pilVWXY1Y2E operon

Cross-References

RefSeq	NP_253244.1
GI	15599750
Affymetrix	PA4554_pilY1_at
Entrez	877859
GenBank	AAG07942.1
INSDC	AAG07942.1
NCBI Locus Tag	PA4554
protein_id(GenBank)	gb|AAG07942.1|AE004869_6|gnl|PseudoCAP|PA4554
TIGR	NTL03PA04555
UniParc	UPI00000C5D60
UniProtKB Acc	Q9HVM8
UniProtKB ID	PILY1_PSEAE
UniRef100	UniRef100_Q9HVM8
UniRef50	UniRef50_S0HPF7
UniRef90	UniRef90_S0HPF7

Product

Feature Type	CDS
Coding Frame	1
Product Name	type 4 fimbrial biogenesis protein PilY1 Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Detected by MALDI-TOF/TOF (PMID:19333994). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-5.09
Kyte-Doolittle Hydrophobicity Value	-0.500
Molecular Weight (kDa)	126.6
Isoelectric Point (pI)	6.35

Subcellular localization

Individual Mappings	Localization Confidence PMID Outer Membrane Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
3HX6	CELL ADHESION	06/19/09	Crystal structure of Pseudomonas aeruginosa PilY1 C-terminal domain	Pseudomonas aeruginosa	2.1	X-RAY DIFFRACTION	98.7

Virulence Evidence

Source 1
Database	VFDB : VF0082
Category	Type IV pili biosynthesis
Evidence
Host Organism
Notes
Reference
Source 2
Database	Victors : 4207
Category	Type IV pili biosynthesis
Evidence	ECO:0000315 mutant phenotype evidence used in manual assertion
Host Organism	Rattus norvegicus
Notes
Infection model	Rat agar bead model of chronic lung infection
Reference	14641575

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 94 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004214 (352 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4554 Search term: pilY1 Search term: type 4 fimbrial biogenesis protein PilY1

Human Homologs

References

Identification of a gene, pilV, required for type 4 fimbrial biogenesis in Pseudomonas aeruginosa, whose product possesses a pre-pilin-like leader sequence. Alm RA, Mattick JS Mol Microbiol 1995 May;16(3):485-96 PubMed ID: 7565109

Genes involved in the biogenesis and function of type-4 fimbriae in Pseudomonas aeruginosa. Alm RA, Mattick JS Gene 1997 Jun 11;192(1):89-98 PubMed ID: 9224878

Pseudomonas aeruginosa AlgR regulates type IV pilus biosynthesis by activating transcription of the fimU-pilVWXY1Y2E operon. Belete B, Lu H, Wozniak DJ J Bacteriol 2008 Mar;190(6):2023-30 PubMed ID: 18178737

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994