Pseudomonas aeruginosa PAO1, PA4555 (pilY2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4555
Name
pilY2
Replicon chromosome
Genomic location 5104170 - 5104517 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
CommentAlgR positively regulates the fimU-pilVWXY1Y2E operon

Cross-References

RefSeq NP_253245.1
GI 15599751
Affymetrix PA4555_pilY2_at
Entrez 878450
GenBank AAG07943.1
INSDC AAG07943.1
NCBI Locus Tag PA4555
protein_id(GenBank) gb|AAG07943.1|AE004869_7|gnl|PseudoCAP|PA4555
TIGR NTL03PA04556
UniParc UPI00000D4268
UniProtKB Acc G3XD55
UniProtKB ID G3XD55_PSEAE
UniRef100 UniRef100_W0YWU2
UniRef50 UniRef50_B7V098
UniRef90 UniRef90_B7V098

Product

Feature Type CDS
Coding Frame 1
Product Name
type 4 fimbrial biogenesis protein PilY2
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -5.56
Kyte-Doolittle Hydrophobicity Value -0.338
Molecular Weight (kDa) 13.0
Isoelectric Point (pI) 4.53

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3TDQ CELL ADHESION 08/11/11 Crystal structure of a fimbrial biogenesis protein PilY2 (PilY2_PA4555) from Pseudomonas aeruginosa PAO1 at 2.10 A resolution Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IV pili biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in one genus

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005157 (259 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4555
Search term: pilY2

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification of a gene, pilV, required for type 4 fimbrial biogenesis in Pseudomonas aeruginosa, whose product possesses a pre-pilin-like leader sequence.
Alm RA, Mattick JS
Mol Microbiol 1995 May;16(3):485-96
PubMed ID: 7565109
Genes involved in the biogenesis and function of type-4 fimbriae in Pseudomonas aeruginosa.
Alm RA, Mattick JS
Gene 1997 Jun 11;192(1):89-98
PubMed ID: 9224878
Pseudomonas aeruginosa AlgR regulates type IV pilus biosynthesis by activating transcription of the fimU-pilVWXY1Y2E operon.
Belete B, Lu H, Wozniak DJ
J Bacteriol 2008 Mar;190(6):2023-30
PubMed ID: 18178737