Pseudomonas aeruginosa PAO1, PA4600 (nfxB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4600
Name
nfxB
Replicon chromosome
Genomic location 5155561 - 5156124 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253290.1
GI 15599796
Affymetrix PA4600_nfxB_at
DNASU PaCD00007993
Entrez 881079
GenBank AAG07988.1
INSDC AAG07988.1
NCBI Locus Tag PA4600
protein_id(GenBank) gb|AAG07988.1|AE004874_1|gnl|PseudoCAP|PA4600
TIGR NTL03PA04601
UniParc UPI000012FFEB
UniProtKB Acc P32265
UniProtKB ID NFXB_PSEAE
UniRef100 UniRef100_P32265
UniRef50 UniRef50_P32265
UniRef90 UniRef90_P32265

Product

Feature Type CDS
Coding Frame 1
Product Name
transcriptional regulator NfxB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.90
Kyte-Doolittle Hydrophobicity Value -0.091
Molecular Weight (kDa) 21.1
Isoelectric Point (pI) 6.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
Type A NfxB Type A NfxB mutants are four to eight times more resistant to ofloxacin, erythromycin, and new zwitterionic cephems, i.e., cefpirome, cefclidin, cefozopran, and cefoselis, than the parent strain, PAO1. nfxB corresponds to 2 loci in Pseudomonas aeruginosa PAO1 (gene name: esrC/nfxB) and 2 loci in Pseudomonas aeruginosa LESB58 (gene name: nfxB). [PMID:8849250] ARO:3004059 protein overexpression model Strict 369.8 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 26 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004257 (359 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4600
Search term: nfxB

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning and nucleotide sequence of the Pseudomonas aeruginosa nfxB gene, conferring resistance to new quinolones.
Okazaki T, Hirai K
FEMS Microbiol Lett 1992 Oct 1;76(1-2):197-202
PubMed ID: 1330820
Purification and characterization of the Pseudomonas aeruginosa NfxB protein, the negative regulator of the nfxB gene.
Shiba T, Ishiguro K, Takemoto N, Koibuchi H, Sugimoto K
J Bacteriol 1995 Oct;177(20):5872-7
PubMed ID: 7592337
Mutation of nfxB causes global changes in the physiology and metabolism of Pseudomonas aeruginosa.
Stickland HG, Davenport PW, Lilley KS, Griffin JL, Welch M
J Proteome Res 2010 Jun 4;9(6):2957-67
PubMed ID: 20373734
Functional characterization of the NfxB repressor of the mexCD-oprJ multidrug efflux operon of Pseudomonas aeruginosa.
Purssell A, Poole K
Microbiology (Reading) 2013 Oct;159(Pt 10):2058-2073
PubMed ID: 23924707