Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4600
|
Name |
nfxB
|
Replicon | chromosome |
Genomic location | 5155561 - 5156124 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_253290.1 |
GI | 15599796 |
Affymetrix | PA4600_nfxB_at |
DNASU | PaCD00007993 |
Entrez | 881079 |
GenBank | AAG07988.1 |
INSDC | AAG07988.1 |
NCBI Locus Tag | PA4600 |
protein_id(GenBank) | gb|AAG07988.1|AE004874_1|gnl|PseudoCAP|PA4600 |
TIGR | NTL03PA04601 |
UniParc | UPI000012FFEB |
UniProtKB Acc | P32265 |
UniProtKB ID | NFXB_PSEAE |
UniRef100 | UniRef100_P32265 |
UniRef50 | UniRef50_P32265 |
UniRef90 | UniRef90_P32265 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
transcriptional regulator NfxB
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -2.90 |
Kyte-Doolittle Hydrophobicity Value | -0.091 |
Molecular Weight (kDa) | 21.1 |
Isoelectric Point (pI) | 6.42 |
Individual Mappings | |
Additional evidence for subcellular localization |
Identified using the Resistance Gene Identifier (RGI)
Model Name | Definition | Accession | Model Type (?) | SNP | Cutoff | Bit Score | Percent Identity | CARD Version |
Type A NfxB | Type A NfxB mutants are four to eight times more resistant to ofloxacin, erythromycin, and new zwitterionic cephems, i.e., cefpirome, cefclidin, cefozopran, and cefoselis, than the parent strain, PAO1. nfxB corresponds to 2 loci in Pseudomonas aeruginosa PAO1 (gene name: esrC/nfxB) and 2 loci in Pseudomonas aeruginosa LESB58 (gene name: nfxB). [PMID:8849250] | ARO:3004059 | protein overexpression model | Strict | 369.8 | 100.0 | 3.2.5 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 26 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004257 (359 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4600
Search term: nfxB
Search term: transcriptional regulator NfxB
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Cloning and nucleotide sequence of the Pseudomonas aeruginosa nfxB gene, conferring resistance to new quinolones.
Okazaki T, Hirai K
FEMS Microbiol Lett 1992 Oct 1;76(1-2):197-202
PubMed ID: 1330820
|
Purification and characterization of the Pseudomonas aeruginosa NfxB protein, the negative regulator of the nfxB gene.
Shiba T, Ishiguro K, Takemoto N, Koibuchi H, Sugimoto K
J Bacteriol 1995 Oct;177(20):5872-7
PubMed ID: 7592337
|
Mutation of nfxB causes global changes in the physiology and metabolism of Pseudomonas aeruginosa.
Stickland HG, Davenport PW, Lilley KS, Griffin JL, Welch M
J Proteome Res 2010 Jun 4;9(6):2957-67
PubMed ID: 20373734
|
Functional characterization of the NfxB repressor of the mexCD-oprJ multidrug efflux operon of Pseudomonas aeruginosa.
Purssell A, Poole K
Microbiology (Reading) 2013 Oct;159(Pt 10):2058-2073
PubMed ID: 23924707
|