Pseudomonas aeruginosa PAO1, PA4601 (morA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4601
Name
morA
Replicon chromosome
Genomic location 5156368 - 5160615 (+ strand)
Transposon Mutants 10 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
Commentabsence of MorA led to a reduction in biofilm formation. MorA is membrane localized.

Cross-References

RefSeq NP_253291.1
GI 15599797
Affymetrix PA4601_at
Entrez 881084
GenBank AAG07989.1
INSDC AAG07989.1
NCBI Locus Tag PA4601
protein_id(GenBank) gb|AAG07989.1|AE004874_2|gnl|PseudoCAP|PA4601
TIGR NTL03PA04602
UniParc UPI00000C5D81
UniProtKB Acc Q9HVI8
UniProtKB ID Q9HVI8_PSEAE
UniRef100 UniRef100_Q9HVI8
UniRef50 UniRef50_Q9HVI8
UniRef90 UniRef90_Q9HVI8

Product

Feature Type CDS
Coding Frame 1
Product Name
motility regulator
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -22.55
Kyte-Doolittle Hydrophobicity Value -0.275
Molecular Weight (kDa) 159.6
Isoelectric Point (pI) 6.01

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4RNF Transferase, Hydrolase 10/24/14 PaMorA tandem diguanylate cyclase - mutant phosphodiesterase, apo form Pseudomonas aeruginosa PAO1 2.85 X-RAY DIFFRACTION 99.3
4RNI HYDROLASE 10/24/14 PaMorA dimeric phosphodiesterase. apo form Pseudomonas aeruginosa PAO1 2.17 X-RAY DIFFRACTION 99.3
4RNJ HYDROLASE 10/24/14 PaMorA phosphodiesterase domain, apo form Pseudomonas aeruginosa PAO1 2.32 X-RAY DIFFRACTION 99.3
4RNH Transferase, Hydrolase 10/24/14 PaMorA tandem diguanylate cyclase - phosphodiesterase, c-di-GMP complex Pseudomonas aeruginosa PAO1 2.45 X-RAY DIFFRACTION 99.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 520 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004258 (973 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4601
Search term: morA

Human Homologs

References

MorA defines a new class of regulators affecting flagellar development and biofilm formation in diverse Pseudomonas species.
Choy WK, Zhou L, Syn CK, Zhang LH, Swarup S
J Bacteriol 2004 Nov;186(21):7221-8
PubMed ID: 15489433
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295