Pseudomonas aeruginosa PAO1, PA4640 (mqoB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4640
Name
mqoB
Replicon chromosome
Genomic location 5208464 - 5209987 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253330.1
GI 15599836
Affymetrix PA4640_mqoB_at
Entrez 881249
GenBank AAG08028.1
NCBI Locus Tag PA4640
protein_id(GenBank) gb|AAG08028.1|AE004878_6|gnl|PseudoCAP|PA4640
TIGR NTL03PA04641
UniProtKB Acc Q9HVF1
UniProtKB ID MQO2_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
malate:quinone oxidoreductase
Product Name Confidence: Class 1
Synonyms malate dehydrogenase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (55.50 kDa, PMID:21751344)
Charge (pH 7) -0.21
Kyte-Doolittle Hydrophobicity Value -0.249
Molecular Weight (kDa) 55.5
Isoelectric Point (pI) 6.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 149 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004293 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4640
Search term: mqoB

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Malate:quinone oxidoreductase is essential for growth on ethanol or acetate in Pseudomonas aeruginosa.
Kretzschmar U, Rückert A, Jeoung JH, Görisch H
Microbiology (Reading) 2002 Dec;148(Pt 12):3839-3847
PubMed ID: 12480887