Pseudomonas aeruginosa PAO1, PA4648 (cupE1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4648
Name
cupE1
Replicon chromosome
Genomic location 5215091 - 5215639 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentThe cupE gene cluster encodes a chaperone usher pathway that is homologous to the A. baumanii csu gene cluster. Activation of the cupE genes leads to the production and assembly of CupE fimbriae on the cell surface. These fimbriae are important in early and late stages of biofilm formation. The expression of the cupE gene cluster is controlled by the two-component system PprA-PprB, and binding of PprB to the cupE1 promoter has been confirmed by EMSA.

Cross-References

RefSeq NP_253337.1
GI 15599843
Affymetrix PA4648_at
Entrez 881285
GenBank AAG08035.1
INSDC AAG08035.1
NCBI Locus Tag PA4648
protein_id(GenBank) gb|AAG08035.1|AE004879_1|gnl|PseudoCAP|PA4648
TIGR NTL03PA04649
UniParc UPI00000C5DA9
UniProtKB Acc Q9HVE4
UniProtKB ID Q9HVE4_PSEAE
UniRef100 UniRef100_W0YZM3
UniRef50 UniRef50_S0HMS8
UniRef90 UniRef90_S0HMS8

Product

Feature Type CDS
Coding Frame 1
Product Name
Pilin subunit CupE1
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -0.67
Kyte-Doolittle Hydrophobicity Value 0.277
Molecular Weight (kDa) 18.5
Isoelectric Point (pI) 6.48

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
8CIO PROTEIN FIBRIL 02/10/23 Cryo-EM structure of the CupE pilus from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 3.5 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 32 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004301 (613 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4648
Search term: cupE1

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The PprA-PprB two-component system activates CupE, the first non-archetypal Pseudomonas aeruginosa chaperone-usher pathway system assembling fimbriae.
Giraud C, Bernard CS, Calderon V, Yang L, Filloux A, Molin S, Fichant G, Bordi C, de Bentzmann S
Environ Microbiol 2011 Mar;13(3):666-83
PubMed ID: 21091863