Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4648
|
Name |
cupE1
|
Replicon | chromosome |
Genomic location | 5215091 - 5215639 (+ strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Comment | The cupE gene cluster encodes a chaperone usher pathway that is homologous to the A. baumanii csu gene cluster. Activation of the cupE genes leads to the production and assembly of CupE fimbriae on the cell surface. These fimbriae are important in early and late stages of biofilm formation. The expression of the cupE gene cluster is controlled by the two-component system PprA-PprB, and binding of PprB to the cupE1 promoter has been confirmed by EMSA. |
RefSeq | NP_253337.1 |
GI | 15599843 |
Affymetrix | PA4648_at |
Entrez | 881285 |
GenBank | AAG08035.1 |
INSDC | AAG08035.1 |
NCBI Locus Tag | PA4648 |
protein_id(GenBank) | gb|AAG08035.1|AE004879_1|gnl|PseudoCAP|PA4648 |
TIGR | NTL03PA04649 |
UniParc | UPI00000C5DA9 |
UniProtKB Acc | Q9HVE4 |
UniProtKB ID | Q9HVE4_PSEAE |
UniRef100 | UniRef100_W0YZM3 |
UniRef50 | UniRef50_S0HMS8 |
UniRef90 | UniRef90_S0HMS8 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
Pilin subunit CupE1
|
Synonyms | |
Evidence for Translation | |
Charge (pH 7) | -0.67 |
Kyte-Doolittle Hydrophobicity Value | 0.277 |
Molecular Weight (kDa) | 18.5 |
Isoelectric Point (pI) | 6.48 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
8CIO | PROTEIN FIBRIL | 02/10/23 | Cryo-EM structure of the CupE pilus from Pseudomonas aeruginosa | Pseudomonas aeruginosa PAO1 | 3.5 | ELECTRON MICROSCOPY | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 32 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004301 (613 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4648
Search term: cupE1
Search term: Pilin subunit CupE1
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
The PprA-PprB two-component system activates CupE, the first non-archetypal Pseudomonas aeruginosa chaperone-usher pathway system assembling fimbriae.
Giraud C, Bernard CS, Calderon V, Yang L, Filloux A, Molin S, Fichant G, Bordi C, de Bentzmann S
Environ Microbiol 2011 Mar;13(3):666-83
PubMed ID: 21091863
|