Pseudomonas aeruginosa PAO1, PA4653 (cupE6)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4653
Name	cupE6
Replicon	chromosome
Genomic location	5219938 - 5220885 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	The cupE gene cluster encodes a chaperone usher pathway that is homologous to the A. baumanii csu gene cluster. Activation of the cupE genes leads to the production and assembly of CupE fimbriae on the cell surface. These fimbriae are important in early and late stages of biofilm formation. The expression of the cupE gene cluster is controlled by the two-component system PprA-PprB, and binding of PprB to the cupE1 promoter has been confirmed by EMSA.

Cross-References

RefSeq	NP_253342.1
GI	15599848
Affymetrix	PA4653_at
DNASU	PaCD00008019
Entrez	881302
GenBank	AAG08040.1
INSDC	AAG08040.1
NCBI Locus Tag	PA4653
protein_id(GenBank)	gb|AAG08040.1|AE004879_6|gnl|PseudoCAP|PA4653
TIGR	NTL03PA04654
UniParc	UPI00000C5DAE
UniProtKB Acc	Q9HVD9
UniProtKB ID	Q9HVD9_PSEAE
UniRef100	UniRef100_W0YZM6
UniRef50	UniRef50_W0YZM6
UniRef90	UniRef90_W0YZM6

Product

Feature Type	CDS
Coding Frame	1
Product Name	Adhesin-like protein CupE6 Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7)	1.15
Kyte-Doolittle Hydrophobicity Value	0.205
Molecular Weight (kDa)	32.5
Isoelectric Point (pI)	7.56

Subcellular localization

Individual Mappings	Localization Confidence PMID Unknown (This protein may have multiple localization sites) Class 3 20472543 Unknown (This protein may have multiple localization sites) Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 41 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004304 (350 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4653 Search term: cupE6 Search term: Adhesin-like protein CupE6

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

The PprA-PprB two-component system activates CupE, the first non-archetypal Pseudomonas aeruginosa chaperone-usher pathway system assembling fimbriae. Giraud C, Bernard CS, Calderon V, Yang L, Filloux A, Molin S, Fichant G, Bordi C, de Bentzmann S Environ Microbiol 2011 Mar;13(3):666-83 PubMed ID: 21091863