Pseudomonas aeruginosa PAO1, PA4664 (prmC)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: hemK
Replicon chromosome
Genomic location 5231653 - 5232483 (- strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_253353.1
GI 15599859
Affymetrix PA4664_hemK_at
Entrez 881353
GenBank AAG08051.1
INSDC AAG08051.1
NCBI Locus Tag PA4664
protein_id(GenBank) gb|AAG08051.1|AE004880_7|gnl|PseudoCAP|PA4664
UniParc UPI00000C5DB7
UniProtKB Acc Q9HVC8
UniRef100 UniRef100_Q9HVC8
UniRef50 UniRef50_Q9HVC8
UniRef90 UniRef90_Q9HVC8


Feature Type CDS
Coding Frame 1
Product Name
S-adenosylmethionine-dependent methyltransferase, PrmC
Product Name Confidence: Class 1
Synonyms hemin biosynthetic protein HemK (misnomer)
probable methyl transferase
Evidence for Translation
Charge (pH 7) -7.37
Kyte-Doolittle Hydrophobicity Value -0.168
Molecular Weight (kDa) 30.5
Isoelectric Point (pI) 5.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 609 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004314 (552 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA4664
Search term: prmC


Cloning and sequencing of a previously unidentified gene that is involved in the biosynthesis of heme in Escherichia coli.
Nakayashiki T, Nishimura K, Inokuchi H
Gene 1995 Feb;153(1):67-70
PubMed ID: 7883187
A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.
Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T
DNA Res. 1996 Jun;3(3):137-55
PubMed ID: 8905232
Functional analysis of the hemK gene product involvement in protoporphyrinogen oxidase activity in yeast.
Le Guen L, Santos R, Camadro JM
FEMS Microbiol. Lett. 1999 Apr;173(1):175-82
PubMed ID: 10220893
The peptide chain release factor methyltransferase PrmC is essential for pathogenicity and environmental adaptation of Pseudomonas aeruginosa PA14.
Pustelny C, Brouwer S, Müsken M, Bielecka A, Dötsch A, Nimtz M, Häussler S
Environ. Microbiol. 2013 Feb;15(2):597-609
PubMed ID: 23278968