Pseudomonas aeruginosa PAO1, PA4665 (prfA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4665
Name
prfA
Synonym: rf1
Replicon chromosome
Genomic location 5232485 - 5233567 (- strand)

Cross-References

RefSeq NP_253354.1
GI 15599860
Affymetrix PA4665_prfA_at
Entrez 881360
GenBank AAG08052.1
INSDC CAA57573.1
NCBI Locus Tag PA4665
protein_id(GenBank) gb|AAG08052.1|AE004880_8|gnl|PseudoCAP|PA4665
TIGR NTL03PA04666
UniParc UPI0000133746
UniProtKB Acc P42806
UniProtKB ID RF1_PSEAE
UniRef100 UniRef100_P42806
UniRef50 UniRef50_Q4K695
UniRef90 UniRef90_P42806

Product

Feature Type CDS
Coding Frame 1
Product Name
peptide chain release factor 1
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.35
Kyte-Doolittle Hydrophobicity Value -0.533
Molecular Weight (kDa) 40.0
Isoelectric Point (pI) 4.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 593 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004315 (562 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4665
Search term: prfA

Human Homologs

Ensembl 86, assembly GRCh38.p7
mitochondrial translational release factor 1 like [Source:HGNC Symbol;Acc:HGNC:21051]
E-value: 2.1e-71
Percent Identity: 47.6

References

Bacterial peptide chain release factors: conserved primary structure and possible frameshift regulation of release factor 2.
Craigen WJ, Cook RG, Tate WP, Caskey CT
Proc. Natl. Acad. Sci. U.S.A. 1985 Jun;82(11):3616-20
PubMed ID: 3889910
Cloning and sequencing of a previously unidentified gene that is involved in the biosynthesis of heme in Escherichia coli.
Nakayashiki T, Nishimura K, Inokuchi H
Gene 1995 Feb;153(1):67-70
PubMed ID: 7883187