Pseudomonas aeruginosa PAO1, PA4668 (lolB)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 5236774 - 5237391 (+ strand)


RefSeq NP_253357.1
GI 15599863
Affymetrix PA4668_at
DNASU PaCD00008025
Entrez 881371
GenBank AAG08055.1
INSDC AAG08055.1
NCBI Locus Tag PA4668
protein_id(GenBank) gb|AAG08055.1|AE004880_11|gnl|PseudoCAP|PA4668
UniParc UPI000012E7C5
UniProtKB Acc P42812
UniRef100 UniRef100_B7V0L4
UniRef50 UniRef50_Q4K692
UniRef90 UniRef90_B7V0L4


Feature Type CDS
Coding Frame 1
Product Name
lipoprotein localization protein LolB
Product Name Confidence: Class 1
Synonyms probable lipoprotein localization protein LolB
Evidence for Translation
Detected using LC-MS/MS (23.03 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 0.93
Kyte-Doolittle Hydrophobicity Value -0.491
Molecular Weight (kDa) 23.0
Isoelectric Point (pI) 7.67

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 2
Outer Membrane Vesicle Class 1
Outer Membrane Class 1
cell fractionation evidence
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 117 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004318 (561 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA4668
Search term: lolB


Regulation of the hemA gene during 5-aminolevulinic acid formation in Pseudomonas aeruginosa.
Hungerer C, Troup B, Römling U, Jahn D
J. Bacteriol. 1995 Mar;177(6):1435-43
PubMed ID: 7883699
A novel outer membrane lipoprotein, LolB (HemM), involved in the LolA (p20)-dependent localization of lipoproteins to the outer membrane of Escherichia coli.
Matsuyama Si, Yokota N, Tokuda H
EMBO J. 1997 Dec;16(23):6947-55
PubMed ID: 9384574
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Characterization of the Pseudomonas aeruginosa Lol system as a lipoprotein sorting mechanism.
Tanaka SY, Narita S, Tokuda H
J. Biol. Chem. 2007 May;282(18):13379-84
PubMed ID: 17350955