Pseudomonas aeruginosa PAO1, PA4670 (prs)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4670
Name
prs
Synonym: prsA
Replicon chromosome
Genomic location 5238408 - 5239349 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_253359.1
GI 15599865
Affymetrix PA4670_prs_at
DNASU PaCD00008027
Entrez 881387
GenBank AAG08057.1
INSDC AAG08057.1
NCBI Locus Tag PA4670
protein_id(GenBank) gb|AAG08057.1|AE004880_13|gnl|PseudoCAP|PA4670
TIGR NTL03PA04671
UniParc UPI000012DFC6
UniProtKB Acc Q9HVC5
UniProtKB ID KPRS_PSEAE
UniRef100 UniRef100_Q9HVC5
UniRef50 UniRef50_Q8PNU0
UniRef90 UniRef90_Q888C6

Product

Feature Type CDS
Coding Frame 1
Product Name
ribose-phosphate pyrophosphokinase
Product Name Confidence: Class 2
Synonyms phosphoribosylpyrophosphate synthetase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (34.04 kDa, PMID:21751344)
Charge (pH 7) -1.67
Kyte-Doolittle Hydrophobicity Value 0.097
Molecular Weight (kDa) 34.0
Isoelectric Point (pI) 6.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 661 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004320 (560 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4670
Search term: prs

Human Homologs

Ensembl 86, assembly GRCh38.p7
phosphoribosyl pyrophosphate synthetase 2 [Source:HGNC Symbol;Acc:HGNC:9465]
E-value: 3.1e-79
Percent Identity: 48.4

References

Phosphoribosylpyrophosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene.
Hove-Jensen B, Harlow KW, King CJ, Switzer RL
J. Biol. Chem. 1986 May;261(15):6765-71
PubMed ID: 3009477
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344