Pseudomonas aeruginosa PAO1, PA4695 (ilvH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4695
Name
ilvH
Replicon chromosome
Genomic location 5273514 - 5274005 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253383.1
GI 15599889
Affymetrix PA4695_ilvH_at
DNASU PaCD00008038
Entrez 881490
GenBank AAG08081.1
INSDC AAG08081.1
NCBI Locus Tag PA4695
protein_id(GenBank) gb|AAG08081.1|AE004883_5|gnl|PseudoCAP|PA4695
TIGR NTL03PA04696
UniParc UPI00000C5DCB
UniProtKB Acc Q9HVA1
UniProtKB ID Q9HVA1_PSEAE
UniRef100 UniRef100_Q9HVA1
UniRef50 UniRef50_A0A086BT49
UniRef90 UniRef90_A0A086BT49

Product

Feature Type CDS
Coding Frame 1
Product Name
acetolactate synthase isozyme III small subunit
Product Name Confidence: Class 2
Synonyms acetohydroxy-acid synthase III small subunit
AHAS-III, ALS-III
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.28
Kyte-Doolittle Hydrophobicity Value 0.079
Molecular Weight (kDa) 17.8
Isoelectric Point (pI) 6.27

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 534 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004344 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4695
Search term: ilvH

Human Homologs

References

Nucleotide sequence of the ilvH-fruR gene region of Escherichia coli K12 and Salmonella typhimurium LT2.
Jahreis K, Postma PW, Lengeler JW
Mol Gen Genet 1991 Apr;226(1-2):332-6
PubMed ID: 1851954
Isolation and characterization of subunits of acetohydroxy acid synthase isozyme III and reconstitution of the holoenzyme.
Vyazmensky M, Sella C, Barak Z, Chipman DM
Biochemistry 1996 Aug 13;35(32):10339-46
PubMed ID: 8756689
Purification of Escherichia coli acetohydroxyacid synthase isoenzyme II and reconstitution of active enzyme from its individual pure subunits.
Hill CM, Pang SS, Duggleby RG
Biochem J 1997 Nov 1;327 ( Pt 3)(Pt 3):891-8
PubMed ID: 9581571