Pseudomonas aeruginosa PAO1, PA4723

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4723
Name
Synonym: dksA1
Replicon chromosome
Genomic location 5302386 - 5302832 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentDksA1 is required for H2O2 tolerance in both planktonic and biofilm growing cells, and protects against macrophage-mediated killing

Cross-References

RefSeq NP_253411.1
GI 15599917
Affymetrix PA4723_dksA_at
Entrez 881594
GenBank AAG08109.1
INSDC AAG08109.1
NCBI Locus Tag PA4723
protein_id(GenBank) gb|AAG08109.1|AE004886_1|gnl|PseudoCAP|PA4723
TIGR NTL03PA04724
UniParc UPI00000C4ED2
UniProtKB Acc G3XD14
UniProtKB ID G3XD14_PSEAE
UniRef100 UniRef100_W0YXF7
UniRef50 UniRef50_P43758
UniRef90 UniRef90_S6BNJ9

Product

Feature Type CDS
Coding Frame 1
Product Name
suppressor protein DksA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected by two-dimensional gel electrophoresis, mass spectral analysis and protein mass mapping (PMID:21863142).
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.0, molecular weight:17.3, PMID:21674802)
Charge (pH 7) -7.40
Kyte-Doolittle Hydrophobicity Value -0.959
Molecular Weight (kDa) 17.3
Isoelectric Point (pI) 4.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 331 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004369 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4723

Human Homologs

References

Inhibition of quorum sensing by a Pseudomonas aeruginosa dksA homologue.
Branny P, Pearson JP, Pesci EC, Köhler T, Iglewski BH, Van Delden C
J Bacteriol 2001 Mar;183(5):1531-9
PubMed ID: 11160083
Nucleotide sequence and characterization of the sfs1 gene: sfs1 is involved in CRP*-dependent mal gene expression in Escherichia coli.
Kawamukai M, Utsumi R, Takeda K, Higashi A, Matsuda H, Choi YL, Komano T
J Bacteriol 1991 Apr;173(8):2644-8
PubMed ID: 2013578
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
Role of a Zn-independent DksA in Zn homeostasis and stringent response.
Blaby-Haas CE, Furman R, Rodionov DA, Artsimovitch I, de Crécy-Lagard V
Mol Microbiol 2011 Feb;79(3):700-15
PubMed ID: 21255113
The Pseudomonas aeruginosa DksA1 protein is involved in H(2)O(2) tolerance and within-macrophages survival and can be replaced by DksA2.
Fortuna A, Collalto D, Schiaffi V, Pastore V, Visca P, Ascenzioni F, Rampioni G, Leoni L
Sci Rep 2022 Jun 21;12(1):10404
PubMed ID: 35729352