Pseudomonas aeruginosa PAO1, PA4812 (fdnG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4812
Name
fdnG
Synonym: fdhG
Replicon chromosome
Genomic location 5398628 - 5401708 (- strand)
Transposon Mutants 4 transposon mutants in PAO1

Cross-References

RefSeq YP_003933614.1
GI 308198350
Affymetrix PA4812_fdnG_at
Entrez 880124
INSDC ABG56538.2
NCBI Locus Tag PA4812
UniParc UPI0000E44C35
UniProtKB Acc Q14T72
UniProtKB ID Q14T72_PSEAE
UniRef100 UniRef100_Q14T72
UniRef50 UniRef50_P24183
UniRef90 UniRef90_Q14T72

Product

Feature Type CDS
Coding Frame 1
Product Name
formate dehydrogenase-O, major subunit
Product Name Confidence: Class 1
Synonyms nitrate-inducible formate dehydrogenase major subunit
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (90.86 kDa, PMID:21751344)
Charge (pH 7) 3.90
Kyte-Doolittle Hydrophobicity Value -0.371
Molecular Weight (kDa) 114010.7
Isoelectric Point (pI) 7.38

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Extracellular Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 497 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004448 (1 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4812
Search term: fdnG

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The purification and properties of formate dehydrogenase and nitrate reductase from Escherichia coli.
Enoch HG, Lester RL
J Biol Chem 1975 Sep 10;250(17):6693-705
PubMed ID: 1099093
Purification and properties of formate dehydrogenase from Pseudomonas aeruginosa. Characterization of haem and iron-sulphur centres by magnetic-circular-dichroism and electron-paramagnetic-resonance spectroscopy.
Godfrey C, Coddington A, Greenwood C, Thomson AJ, Gadsby PM
Biochem J 1987 Apr 1;243(1):225-33
PubMed ID: 3038081
Purification and properties of formate dehydrogenase from Pseudomonas aeruginosa. Electron-paramagnetic-resonance studies on the molybdenum centre.
Gadsby PM, Greenwood C, Coddington A, Thomson AJ, Godfrey C
Biochem J 1987 Apr 1;243(1):235-9
PubMed ID: 3038082
Electron-paramagnetic-resonance and magnetic-circular-dichroism studies on the formate dehydrogenase-nitrate reductase particle from Pseudomonas aeruginosa.
Godfrey C, Gadsby PM, Thomson AJ, Greenwood C, Coddington A
Biochem J 1987 Apr 1;243(1):241-8
PubMed ID: 3038083
Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. I. Nucleotide sequence of the fdnGHI operon and evidence that opal (UGA) encodes selenocysteine.
Berg BL, Li J, Heider J, Stewart V
J Biol Chem 1991 Nov 25;266(33):22380-5
PubMed ID: 1834669
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344