Pseudomonas aeruginosa PAO1, PA4837 (cntO)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4837
Name
cntO
Synonym: TonB-dependent Outer Membrane Protein ZrmA
Synonym: zrmA
Replicon chromosome
Genomic location 5427716 - 5429842 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 13 transposon mutants in orthologs
Commentpart of the Zur-regulated metallophore operon known as cntOLMI (PMID:29214991) or zrmABCD(PMID:28898501).

Cross-References

RefSeq NP_253524.1
GI 15600030
Affymetrix PA4837_at
Entrez 878137
GenBank AAG08222.1
INSDC AAG08222.1
NCBI Locus Tag PA4837
protein_id(GenBank) gb|AAG08222.1|AE004896_12|gnl|PseudoCAP|PA4837
TIGR NTL03PA04838
UniParc UPI00000C5E32
UniProtKB Acc Q9HUX3
UniProtKB ID Q9HUX3_PSEAE
UniRef100 UniRef100_Q9HUX3
UniRef50 UniRef50_Q9HUX3
UniRef90 UniRef90_Q9HUX3

Product

Feature Type CDS
Coding Frame 1
Product Name
CntO
Product Name Confidence: Class 1
Synonyms probable outer membrane protein precursor
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -12.14
Kyte-Doolittle Hydrophobicity Value -0.582
Molecular Weight (kDa) 79.1
Isoelectric Point (pI) 5.67

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 302 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000149 (2669 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4837
Search term: cntO
Search term: CntO

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Growth of Pseudomonas aeruginosa in zinc poor environments is promoted by a nicotianamine-related metallophore.
Mastropasqua MC, D'Orazio M, Cerasi M, Pacello F, Gismondi A, Canini A, Canuti L, Consalvo A, Ciavardelli D, Chirullo B, Pasquali P, Battistoni A
Mol Microbiol 2017 Nov;106(4):543-561
PubMed ID: 28898501
Pseudomonas aeruginosa zinc uptake in chelating environment is primarily mediated by the metallophore pseudopaline.
Lhospice S, Gomez NO, Ouerdane L, Brutesco C, Ghssein G, Hajjar C, Liratni A, Wang S, Richaud P, Bleves S, Ball G, Borezée-Durant E, Lobinski R, Pignol D, Arnoux P, Voulhoux R
Sci Rep 2017 Dec 7;7(1):17132
PubMed ID: 29214991