Pseudomonas aeruginosa PAO1, PA4843 (gcbA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4843
Name
gcbA
Replicon chromosome
Genomic location 5436079 - 5437707 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253530.1
GI 15600036
Affymetrix PA4843_at
Entrez 879632
GenBank AAG08228.1
INSDC AAG08228.1
NCBI Locus Tag PA4843
protein_id(GenBank) gb|AAG08228.1|AE004897_6|gnl|PseudoCAP|PA4843
TIGR NTL03PA04844
UniParc UPI00000C5E37
UniProtKB Acc Q9HUW7
UniProtKB ID Q9HUW7_PSEAE
UniRef100 UniRef100_W0Z042
UniRef50 UniRef50_G8Q613
UniRef90 UniRef90_B7V1Q5

Product

Feature Type CDS
Coding Frame 1
Product Name
GcbA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.75
Kyte-Doolittle Hydrophobicity Value -0.406
Molecular Weight (kDa) 61.6
Isoelectric Point (pI) 6.34

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 601 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004473 (530 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4843
Search term: gcbA
Search term: GcbA

Human Homologs

References

Identification of a novel response regulator required for the swarmer-to-stalked-cell transition in Caulobacter crescentus.
Hecht GB, Newton A
J Bacteriol 1995 Nov;177(21):6223-9
PubMed ID: 7592388
ChIP-Seq and RNA-Seq reveal an AmrZ-mediated mechanism for cyclic di-GMP synthesis and biofilm development by Pseudomonas aeruginosa.
Jones CJ, Newsom D, Kelly B, Irie Y, Jennings LK, Xu B, Limoli DH, Harrison JJ, Parsek MR, White P, Wozniak DJ
PLoS Pathog 2014 Mar;10(3):e1003984
PubMed ID: 24603766
The Pseudomonas aeruginosa diguanylate cyclase GcbA, a homolog of P. fluorescens GcbA, promotes initial attachment to surfaces, but not biofilm formation, via regulation of motility.
Petrova OE, Cherny KE, Sauer K
J Bacteriol 2014 Aug;196(15):2827-41
PubMed ID: 24891445
The diguanylate cyclase GcbA facilitates Pseudomonas aeruginosa biofilm dispersion by activating BdlA.
Petrova OE, Cherny KE, Sauer K
J Bacteriol 2015 Jan 1;197(1):174-87
PubMed ID: 25331436