Pseudomonas aeruginosa PAO1, PA4853 (fis)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4853
Name
fis
Replicon chromosome
Genomic location 5448643 - 5448966 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253540.1
GI 15600046
Affymetrix PA4853_fis_at
Entrez 880045
GenBank AAG08238.1
INSDC AAG08238.1
NCBI Locus Tag PA4853
protein_id(GenBank) gb|AAG08238.1|AE004898_9|gnl|PseudoCAP|PA4853
TIGR NTL03PA04854
UniParc UPI000012A7CF
UniProtKB Acc Q9HUW0
UniProtKB ID FISL_PSEAE
UniRef100 UniRef100_Q9HUW0
UniRef50 UniRef50_Q9HUW0
UniRef90 UniRef90_Q9HUW0

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA-binding protein Fis
Product Name Confidence: Class 2
Evidence for Translation
Charge (pH 7) -1.08
Kyte-Doolittle Hydrophobicity Value -0.439
Molecular Weight (kDa) 11.9
Isoelectric Point (pI) 6.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 198 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004482 (560 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4853
Search term: fis

Transposon Insertions in PAO1

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_64190
Mutant ID PA14NR: 29775
Genomic Position 5719630
Position in ORF 323(324)
Reference 16477005

References

The transactivation region of the fis protein that controls site-specific DNA inversion contains extended mobile beta-hairpin arms.
Safo MK, Yang WZ, Corselli L, Cramton SE, Yuan HS, Johnson RC
EMBO J. 1997 Nov;16(22):6860-73
PubMed ID: 9362499
Fis Regulates Type III Secretion System by Influencing the Transcription of exsA in Pseudomonas aeruginosa Strain PA14.
Deng X, Li M, Pan X, Zheng R, Liu C, Chen F, Liu X, Cheng Z, Jin S, Wu W
Front Microbiol 2017 ;8
PubMed ID: 28469612