Pseudomonas aeruginosa PAO1, PA4878 (brlR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4878
Name
brlR
Replicon chromosome
Genomic location 5473766 - 5474578 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253565.1
GI 15600071
Affymetrix PA4878_at
DNASU PaCD00008112
Entrez 882234
GenBank AAG08263.1
INSDC AAG08263.1
NCBI Locus Tag PA4878
protein_id(GenBank) gb|AAG08263.1|AE004901_5|gnl|PseudoCAP|PA4878
TIGR NTL03PA04879
UniParc UPI00000C5E54
UniProtKB Acc Q9HUT5
UniProtKB ID Q9HUT5_PSEAE
UniRef100 UniRef100_W0YYW8
UniRef50 UniRef50_A6VCY6
UniRef90 UniRef90_W0YYW8

Product

Feature Type CDS
Coding Frame 1
Product Name
BrlR
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.39
Kyte-Doolittle Hydrophobicity Value -0.451
Molecular Weight (kDa) 31.2
Isoelectric Point (pI) 6.92

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5XBW DNA BINDING PROTEIN 03/21/17 The structure of BrlR Pseudomonas aeruginosa PAO1 3.109 X-RAY DIFFRACTION 100.0
5XBT DNA BINDING PROTEIN 03/21/17 The structure of BrlR bound to c-di-GMP Pseudomonas aeruginosa PAO1 2.495 X-RAY DIFFRACTION 100.0
5YC9 TRANSCRIPTION 09/07/17 Crystal structure of a Pseudomonas aeruginosa transcriptional regulator Pseudomonas aeruginosa 2.9 X-RAY DIFFRACTION 100.0
5XBI TRANSCRIPTION 03/17/17 The structure of BrlR-C domain bound to 3-amino-2-phenazino(a pyocyanin analog) Pseudomonas aeruginosa 1.4 X-RAY DIFFRACTION 99.3
5XQL TRANSCRIPTION 06/07/17 Crystal structure of a Pseudomonas aeruginosa transcriptional regulator Pseudomonas aeruginosa 2.494 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 235 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004506 (322 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4878
Search term: brlR
Search term: BrlR

Human Homologs

References

The MerR-like transcriptional regulator BrlR contributes to Pseudomonas aeruginosa biofilm tolerance.
Liao J, Sauer K
J Bacteriol 2012 Sep;194(18):4823-36
PubMed ID: 22730129
The MerR-like regulator BrlR confers biofilm tolerance by activating multidrug efflux pumps in Pseudomonas aeruginosa biofilms.
Liao J, Schurr MJ, Sauer K
J Bacteriol 2013 Aug;195(15):3352-63
PubMed ID: 23687276
The MerR-like regulator BrlR impairs Pseudomonas aeruginosa biofilm tolerance to colistin by repressing PhoPQ.
Chambers JR, Sauer K
J Bacteriol 2013 Oct;195(20):4678-88
PubMed ID: 23935054