Pseudomonas aeruginosa PAO1, PA4914 (amaR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4914
Name
amaR
Replicon chromosome
Genomic location 5513732 - 5514670 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253601.1
GI 15600107
Affymetrix PA4914_at
Entrez 879725
GenBank AAG08299.1
INSDC AAG08299.1
NCBI Locus Tag PA4914
protein_id(GenBank) gb|AAG08299.1|AE004904_7|gnl|PseudoCAP|PA4914
TIGR NTL03PA04915
UniParc UPI00000C5E78
UniProtKB Acc Q9HUP9
UniProtKB ID Q9HUP9_PSEAE
UniRef100 UniRef100_W0YYY9
UniRef50 UniRef50_Q48NY0
UniRef90 UniRef90_A6VD26

Product

Feature Type CDS
Coding Frame 1
Product Name
transcriptional regulator, AmaR
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.35
Kyte-Doolittle Hydrophobicity Value -0.222
Molecular Weight (kDa) 35.3
Isoelectric Point (pI) 6.94

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 494 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000439 (1080 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4914
Search term: amaR

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 48760
Genomic Position 5513980
Position in ORF 249(939)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 1875
Genomic Position 5514128
Position in ORF 397(939)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_64910
Mutant ID PA14NR: 35296
Genomic Position 5785582
Position in ORF 483(939)
Reference 16477005

References

Molecular characterization of lysR-lysXE, gcdR-gcdHG and amaR-amaAB operons for lysine export and catabolism: a comprehensive lysine catabolic network in Pseudomonas aeruginosa PAO1.
Madhuri Indurthi S(1), Chou HT(1), Lu CD(2).
Microbiology. 2016 May;162(5):876-88.
PubMed ID: 26967762