Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4974
|
Name |
Synonym: opmH |
Replicon | chromosome |
Genomic location | 5584101 - 5585549 (+ strand) |
Transposon Mutants | 4 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Comment | Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878). |
RefSeq | NP_253661.1 |
GI | 15600167 |
Affymetrix | PA4974_at |
Entrez | 879703 |
GenBank | AAG08359.1 |
INSDC | AAG08359.1 |
NCBI Locus Tag | PA4974 |
protein_id(GenBank) | gb|AAG08359.1|AE004910_6|gnl|PseudoCAP|PA4974 |
TIGR | NTL03PA04975 |
UniParc | UPI0000001FDB |
UniProtKB Acc | Q9HUJ1 |
UniProtKB ID | Q9HUJ1_PSEAE |
UniRef100 | UniRef100_G3XCK3 |
UniRef50 | UniRef50_G3XCK3 |
UniRef90 | UniRef90_G3XCK3 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
probable outer membrane protein precursor
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using 2-DE, MALDI-TOF/MS analysis (53.41 kDa, pI 5.8, PMID:16335974)
Detected using LC-MS/MS (53.41 kDa, PMID:21751344)
|
Charge (pH 7) | -3.02 |
Kyte-Doolittle Hydrophobicity Value | -0.518 |
Molecular Weight (kDa) | 53.4 |
Isoelectric Point (pI) | 5.88 |
Individual Mappings | |
Additional evidence for subcellular localization |
Detected in sarcosine-insoluble outer membrane fraction using 2-DE, MALDI-TOF/MS analysis (PMID:16335974)
|
Identified using the Resistance Gene Identifier (RGI)
Model Name | Definition | Accession | Model Type (?) | SNP | Cutoff | Bit Score | Percent Identity | CARD Version |
OpmH | OpmH is an outer membrane efflux protein required for triclosan-specific efflux pump function. [PMID:15855547, PMID:17720796] | ARO:3003682 | protein homolog model | Perfect | 914.8 | 100.0 | 3.2.5 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 300 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004589 (534 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4974
Search term: probable outer membrane protein precursor
|
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
|
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878
|