Pseudomonas aeruginosa PAO1, PA4976 (aruH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4976
Name
aruH
Synonym: aruH
Replicon chromosome
Genomic location 5586370 - 5587551 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253663.1
GI 15600169
Affymetrix PA4976_aspC_at
Entrez 880915
GenBank AAG08361.1
INSDC AAG08361.1
NCBI Locus Tag PA4976
protein_id(GenBank) gb|AAG08361.1|AE004910_8|gnl|PseudoCAP|PA4976
TIGR NTL03PA04977
UniParc UPI00000C5EA5
UniProtKB Acc Q9HUI9
UniProtKB ID ARUH_PSEAE
UniRef100 UniRef100_Q9HUI9
UniRef50 UniRef50_Q9HUI9
UniRef90 UniRef90_Q9HUI9

Product

Feature Type CDS
Coding Frame 1
Product Name
Arginine:Pyruvate Transaminas, AruH
Product Name Confidence: Class 1
Synonyms ASPAT
Evidence for Translation
Charge (pH 7) -9.89
Kyte-Doolittle Hydrophobicity Value 0.072
Molecular Weight (kDa) 42.4
Isoelectric Point (pI) 5.27

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 652 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004590 (439 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4976
Search term: aruH

Human Homologs

Ensembl 110, assembly GRCh38.p14
kynurenine aminotransferase 3 [Source:HGNC Symbol;Acc:HGNC:33238]
E-value: 7.2e-38
Percent Identity: 30.2
Ensembl 110, assembly GRCh38.p14
kynurenine aminotransferase 3 [Source:HGNC Symbol;Acc:HGNC:33238]
E-value: 7.2e-38
Percent Identity: 30.2

References

Cloning and nucleotide sequencing of Rhizobium meliloti aminotransferase genes: an aspartate aminotransferase required for symbiotic nitrogen fixation is atypical.
Watson RJ, Rastogi VK
J Bacteriol 1993 Apr;175(7):1919-28
PubMed ID: 8096210
Stereospecific production of the herbicide phosphinothricin (glufosinate): purification of aspartate transaminase from Bacillus stearothermophilus, cloning of the corresponding gene, aspC, and application in a coupled transaminase process.
Bartsch K, Schneider R, Schulz A
Appl Environ Microbiol 1996 Oct;62(10):3794-9
PubMed ID: 8837436
Functional genomics enables identification of genes of the arginine transaminase pathway in Pseudomonas aeruginosa.
Yang Z, Lu CD
J Bacteriol 2007 Jun;189(11):3945-53
PubMed ID: 17416670
Characterization of an arginine:pyruvate transaminase in arginine catabolism of Pseudomonas aeruginosa PAO1.
Yang Z, Lu CD
J Bacteriol 2007 Jun;189(11):3954-9
PubMed ID: 17416668