Pseudomonas aeruginosa PAO1, PA4981 (lysP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4981
Name
lysP
Replicon chromosome
Genomic location 5593683 - 5595095 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 10 transposon mutants in orthologs

Cross-References

RefSeq NP_253668.1
GI 15600174
Affymetrix PA4981_at
Entrez 880427
GenBank AAG08366.1
INSDC AAG08366.1
NCBI Locus Tag PA4981
protein_id(GenBank) gb|AAG08366.1|AE004911_1|gnl|PseudoCAP|PA4981
TIGR NTL03PA04982
UniParc UPI00000C5EAA
UniProtKB Acc Q9HUI4
UniProtKB ID Q9HUI4_PSEAE
UniRef100 UniRef100_Q9HUI4
UniRef50 UniRef50_P39137
UniRef90 UniRef90_Q9HUI4

Product

Feature Type CDS
Coding Frame 1
Product Name
lysine-specific permease
Product Name Confidence: Class 1
Synonyms probable amino acid permease
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 8.49
Kyte-Doolittle Hydrophobicity Value 0.840
Molecular Weight (kDa) 50.6
Isoelectric Point (pI) 9.11

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 395 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000219 (3043 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4981
Search term: lysP

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Expression of the rocDEF operon involved in arginine catabolism in Bacillus subtilis.
Gardan R, Rapoport G, Débarbouillé M
J Mol Biol 1995 Jun 23;249(5):843-56
PubMed ID: 7540694
Molecular characterization of lysR-lysXE, gcdR-gcdHG and amaR-amaAB operons for lysine export and catabolism: a comprehensive lysine catabolic network in Pseudomonas aeruginosa PAO1.
Madhuri Indurthi S, Chou HT, Lu CD
Microbiology (Reading) 2016 May;162(5):876-888
PubMed ID: 26967762
Cloning, expression, purification, crystallization and preliminary X-ray diffraction of a lysine-specific permease from Pseudomonas aeruginosa.
Nji E, Li D, Doyle DA, Caffrey M
Acta Crystallogr F Struct Biol Commun 2014 Oct;70(Pt 10):1362-7
PubMed ID: 25286940