Pseudomonas aeruginosa PAO1, PA4981 (lysP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4981
Name
lysP
Replicon chromosome
Genomic location 5593683 - 5595095 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253668.1
GI 15600174
Affymetrix PA4981_at
Entrez 880427
GenBank AAG08366.1
INSDC AAG08366.1
NCBI Locus Tag PA4981
protein_id(GenBank) gb|AAG08366.1|AE004911_1|gnl|PseudoCAP|PA4981
TIGR NTL03PA04982
UniParc UPI00000C5EAA
UniProtKB Acc Q9HUI4
UniProtKB ID Q9HUI4_PSEAE
UniRef100 UniRef100_Q9HUI4
UniRef50 UniRef50_P39137
UniRef90 UniRef90_Q9HUI4

Product

Feature Type CDS
Coding Frame 1
Product Name
lysine-specific permease
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 8.49
Kyte-Doolittle Hydrophobicity Value 0.840
Molecular Weight (kDa) 50.6
Isoelectric Point (pI) 9.11

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 395 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000219 (3173 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4981
Search term: lysP

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 35240
Genomic Position 5594053
Position in ORF 371(1413)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_15_H10
Genomic Position 5595005
Position in ORF 1323(1413)
Reference 15805499
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 51407
Genomic Position 5594942
Position in ORF 1260(1413)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_97_A1
Genomic Position 5595005
Position in ORF 1323(1413)
Reference 15805499

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_65850
Mutant ID PA14NR: 41049
Genomic Position 5866339
Position in ORF 1260(1413)
Reference 16477005

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PubMed ID: 15687295
Expression of the rocDEF operon involved in arginine catabolism in Bacillus subtilis.
Gardan R, Rapoport G, Debarbouille M.
J Mol Biol. 1995 Jun 23;249(5):843-56.
PubMed ID: 7540694
Molecular characterization of lysR-lysXE, gcdR-gcdHG and amaR-amaAB operons for lysine export and catabolism: a comprehensive lysine catabolic network in Pseudomonas aeruginosa PAO1.
Madhuri Indurthi S(1), Chou HT(1), Lu CD(2).
Microbiology. 2016 May;162(5):876-88.
PubMed ID: 26967762
Cloning, expression, purification, crystallization and preliminary X-ray diffraction of a lysine-specific permease from Pseudomonas aeruginosa.
Nji E(1), Li D(1), Doyle DA(1), Caffrey M(1).
Acta Crystallogr F Struct Biol Commun. 2014 Oct;70(Pt 10):1362-7.
PubMed ID: 25286940