Pseudomonas aeruginosa PAO1, PA4997 (msbA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4997
Name
msbA
Replicon chromosome
Genomic location 5613733 - 5615544 (- strand)
CommentmsbA Is Essential to the Viability of P. aeruginosa

Cross-References

RefSeq NP_253684.1
GI 15600190
Affymetrix PA4997_msbA_at
Entrez 881672
GenBank AAG08382.1
INSDC AAG08382.1
NCBI Locus Tag PA4997
protein_id(GenBank) gb|AAG08382.1|AE004912_8|gnl|PseudoCAP|PA4997
TIGR NTL03PA04998
UniParc UPI00000C5EBA
UniProtKB Acc Q9HUG8
UniProtKB ID MSBA_PSEAE
UniRef100 UniRef100_Q9HUG8
UniRef50 UniRef50_Q9HUG8
UniRef90 UniRef90_Q9HUG8

Product

Feature Type CDS
Coding Frame 1
Product Name
transport protein MsbA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (66.43 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.09
Kyte-Doolittle Hydrophobicity Value 0.153
Molecular Weight (kDa) 66.4
Isoelectric Point (pI) 6.98

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004607 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4997
Search term: msbA

Human Homologs

Ensembl 110, assembly GRCh38.p14
ATP binding cassette subfamily B member 9 [Source:HGNC Symbol;Acc:HGNC:50]
E-value: 1.6e-73
Percent Identity: 33.9
Ensembl 110, assembly GRCh38.p14
ATP binding cassette subfamily B member 9 [Source:HGNC Symbol;Acc:HGNC:50]
E-value: 1.6e-73
Percent Identity: 33.9
Ensembl 110, assembly GRCh38.p14
ATP binding cassette subfamily B member 9 [Source:HGNC Symbol;Acc:HGNC:50]
E-value: 1.6e-73
Percent Identity: 33.9

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Sequence of the rec-2 locus of Haemophilus influenzae: homologies to comE-ORF3 of Bacillus subtilis and msbA of Escherichia coli.
Clifton SW, McCarthy D, Roe BA
Gene 1994 Aug 19;146(1):95-100
PubMed ID: 8063112
Function of Escherichia coli MsbA, an essential ABC family transporter, in lipid A and phospholipid biosynthesis.
Zhou Z, White KA, Polissi A, Georgopoulos C, Raetz CR
J Biol Chem 1998 May 15;273(20):12466-75
PubMed ID: 9575204
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Biochemical characterization of MsbA from Pseudomonas aeruginosa.
Ghanei H, Abeyrathne PD, Lam JS
J Biol Chem 2007 Sep 14;282(37):26939-26947
PubMed ID: 17644517