Pseudomonas aeruginosa PAO1, PA4999 (waaL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4999
Name
waaL
Replicon chromosome
Genomic location 5616310 - 5617515 (+ strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentWaaL is important for cell wall integrity and motility of the bacteria.

Cross-References

RefSeq NP_253686.1
GI 15600192
Affymetrix PA4999_at
Entrez 881661
GenBank AAG08384.1
INSDC AAG08384.1
NCBI Locus Tag PA4999
protein_id(GenBank) gb|AAG08384.1|AE004912_10|gnl|PseudoCAP|PA4999
TIGR NTL03PA05000
UniParc UPI00000C5EBC
UniProtKB Acc Q9HUG6
UniProtKB ID PA99_PSEAE
UniRef100 UniRef100_Q9HUG6
UniRef50 UniRef50_Q9HUG6
UniRef90 UniRef90_Q9HUG6

Product

Feature Type CDS
Coding Frame 1
Product Name
O-antigen ligase, WaaL
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 15.11
Kyte-Doolittle Hydrophobicity Value 0.628
Molecular Weight (kDa) 44.4
Isoelectric Point (pI) 10.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 15 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004608 (840 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4999
Search term: waaL

Human Homologs

References

Functional characterization of WaaL, a ligase associated with linking O-antigen polysaccharide to the core of Pseudomonas aeruginosa lipopolysaccharide.
Abeyrathne PD, Daniels C, Poon KK, Matewish MJ, Lam JS
J Bacteriol 2005 May;187(9):3002-12
PubMed ID: 15838026
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
WaaL of Pseudomonas aeruginosa utilizes ATP in in vitro ligation of O antigen onto lipid A-core.
Abeyrathne PD, Lam JS
Mol Microbiol 2007 Sep;65(5):1345-59
PubMed ID: 17697256