Pseudomonas aeruginosa PAO1, PA5002 (dnpA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5002
Name
dnpA
Replicon chromosome
Genomic location 5619608 - 5621026 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
Comment

Cross-References

RefSeq NP_253689.1
GI 15600195
Affymetrix PA5002_at
Entrez 881666
GenBank AAG08387.1
INSDC AAG08387.1
NCBI Locus Tag PA5002
protein_id(GenBank) gb|AAG08387.1|AE004913_2|gnl|PseudoCAP|PA5002
TIGR NTL03PA05003
UniParc UPI00000C5EBF
UniProtKB Acc Q9HUG3
UniProtKB ID Q9HUG3_PSEAE
UniRef100 UniRef100_Q9HUG3
UniRef50 UniRef50_Q87VE8
UniRef90 UniRef90_Q9HUG3

Product

Feature Type CDS
Coding Frame 1
Product Name
de-N-acetylase involved in persistence, DnpA
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 6.99
Kyte-Doolittle Hydrophobicity Value -0.270
Molecular Weight (kDa) 53.6
Isoelectric Point (pI) 8.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 9 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004610 (515 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5002
Search term: dnpA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
A putative de-N-acetylase of the PIG-L superfamily affects fluoroquinolone tolerance in Pseudomonas aeruginosa.
Liebens V, Defraine V, Van der Leyden A, De Groote VN, Fierro C, Beullens S, Verstraeten N, Kint C, Jans A, Frangipani E, Visca P, Marchal K, Versées W, Fauvart M, Michiels J
Pathog Dis 2014 Jun;71(1):39-54
PubMed ID: 24692291
The Putative De-N-acetylase DnpA Contributes to Intracellular and Biofilm-Associated Persistence of Pseudomonas aeruginosa Exposed to Fluoroquinolones.
Khandekar S, Liebens V, Fauvart M, Tulkens PM, Michiels J, Van Bambeke F
Front Microbiol 2018;9:1455
PubMed ID: 30042739