Pseudomonas aeruginosa PAO1, PA5009 (waaP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5009
Name
waaP
Synonym: rfaP
Replicon chromosome
Genomic location 5627865 - 5628671 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_253696.1
GI 15600202
Affymetrix PA5009_waaP_at
DNASU PaCD00008162
Entrez 881540
GenBank AAG08394.1
INSDC AAG08394.1
NCBI Locus Tag PA5009
protein_id(GenBank) gb|AAG08394.1|AE004913_9|gnl|PseudoCAP|PA5009
TIGR NTL03PA05010
UniParc UPI00000C5EC5
UniProtKB Acc Q9HUF7
UniProtKB ID RFAP_PSEAE
UniRef100 UniRef100_Q9HUF7
UniRef50 UniRef50_Q9HUF7
UniRef90 UniRef90_Q9HUF7

Product

Feature Type CDS
Coding Frame 1
Product Name
lipopolysaccharide kinase WaaP
Product Name Confidence: Class 1
Synonyms RfaP protein
heptose kinase WaaP
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 13.87
Kyte-Doolittle Hydrophobicity Value -0.553
Molecular Weight (kDa) 31.3
Isoelectric Point (pI) 10.37

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6DFL HYDROLASE 05/15/18 WaaP in complex with acyl carrier protein Escherichia coli; Pseudomonas aeruginosa 2.396 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 25 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004616 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5009
Search term: waaP

Human Homologs

References

WaaP of Pseudomonas aeruginosa is a novel eukaryotic type protein-tyrosine kinase as well as a sugar kinase essential for the biosynthesis of core lipopolysaccharide.
Zhao X, Lam JS
J Biol Chem 2002 Feb 15;277(7):4722-30
PubMed ID: 11741974
[How signal transduction systems look like after recent dramatic progress].
Chida K, Fukui Y, Kato S, Itoh H
Tanpakushitsu Kakusan Koso 1999 Dec;44(16):2535-64
PubMed ID: 10589288
Purification and characterization of WaaP from Escherichia coli, a lipopolysaccharide kinase essential for outer membrane stability.
Yethon JA, Whitfield C
J Biol Chem 2001 Feb 23;276(8):5498-504
PubMed ID: 11069912
Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12.
Parker CT, Pradel E, Schnaitman CA
J Bacteriol 1992 Feb;174(3):930-4
PubMed ID: 1732225
Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica.
Heinrichs DE, Yethon JA, Whitfield C
Mol Microbiol 1998 Oct;30(2):221-32
PubMed ID: 9791168