Pseudomonas aeruginosa PAO1, PA5010 (waaG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5010
Name
waaG
Synonym: rfaG
Replicon chromosome
Genomic location 5628668 - 5629789 (- strand)

Cross-References

RefSeq NP_253697.1
GI 15600203
Affymetrix PA5010_waaG_at
Entrez 881532
GenBank AAG08395.1
INSDC AAG08395.1
NCBI Locus Tag PA5010
protein_id(GenBank) gb|AAG08395.1|AE004913_10|gnl|PseudoCAP|PA5010
TIGR NTL03PA05011
UniParc UPI00000C5EC6
UniProtKB Acc Q9HUF6
UniProtKB ID Q9HUF6_PSEAE
UniRef100 UniRef100_Q9HUF6
UniRef50 UniRef50_G8Q3T6
UniRef90 UniRef90_Q9HUF6

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-glucose:(heptosyl) LPS alpha 1,3-glucosyltransferase WaaG
Product Name Confidence: Class 1
Synonyms Glucosyltransferase I
LPS core biosynthetic gene WaaG
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.84
Kyte-Doolittle Hydrophobicity Value -0.199
Molecular Weight (kDa) 42.2
Isoelectric Point (pI) 7.65

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 403 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004617 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5010
Search term: waaG

Human Homologs

References

Identification by Tn10 transposon mutagenesis of host factors involved in the biosynthesis of K99 fimbriae of Escherichia coli: effect of LPS core mutations.
Pilipcinec E, Huisman TT, Willemsen PT, Appelmelk BJ, de Graaf FK, Oudega B
FEMS Microbiol Lett 1994 Oct 15;123(1-2):201-6
PubMed ID: 7988890
Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica.
Heinrichs DE, Yethon JA, Whitfield C
Mol Microbiol 1998 Oct;30(2):221-32
PubMed ID: 9791168
Cloning and targeted disruption of two lipopolysaccharide biosynthesis genes, kdsA and waaG, of Pseudomonas aeruginosa PAO1 by site-directed mutagenesis.
Perumal D, Sakharkar KR, Tang TH, Chow VT, Lim CS, Samal A, Sugiura N, Sakharkar MK
J Mol Microbiol Biotechnol 2010;19(4):169-79
PubMed ID: 21042030