Pseudomonas aeruginosa PAO1, PA5040 (pilQ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5040
Name
pilQ
Replicon chromosome
Genomic location 5675714 - 5677858 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 5 transposon mutants in orthologs

Cross-References

RefSeq NP_253727.1
GI 15600233
Affymetrix PA5040_pilQ_at
Entrez 880962
GenBank AAG08425.1
INSDC AAG08425.1
NCBI Locus Tag PA5040
protein_id(GenBank) gb|AAG08425.1|AE004917_4|gnl|PseudoCAP|PA5040
TIGR NTL03PA05041
UniParc UPI0000131ACD
UniProtKB Acc P34750
UniProtKB ID PILQ_PSEAE
UniRef100 UniRef100_P34750
UniRef50 UniRef50_P34750
UniRef90 UniRef90_P34750

Product

Feature Type CDS
Coding Frame 1
Product Name
Type 4 fimbrial biogenesis outer membrane protein PilQ precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (77.38 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.77
Kyte-Doolittle Hydrophobicity Value -0.257
Molecular Weight (kDa) 77.4
Isoelectric Point (pI) 5.23

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 1
Outer Membrane Vesicle Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6VE2 PROTEIN TRANSPORT 12/28/19 Tetradecameric PilQ bound by TsaP heptamer from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 4.3 ELECTRON MICROSCOPY 95.3
6VE4 PROTEIN TRANSPORT 12/28/19 Pentadecameric PilQ from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 6.9 ELECTRON MICROSCOPY 95.3
6VE3 PROTEIN TRANSPORT 12/28/19 Tetradecameric PilQ from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 4.3 ELECTRON MICROSCOPY 95.3

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IV pili biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 346 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000664 (1798 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5040
Search term: pilQ

Human Homologs

References

Formation of oligomeric rings by XcpQ and PilQ, which are involved in protein transport across the outer membrane of Pseudomonas aeruginosa.
Bitter W, Koster M, Latijnhouwers M, de Cock H, Tommassen J
Mol Microbiol 1998 Jan;27(1):209-19
PubMed ID: 9466268
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Characterization of pilQ, a new gene required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa.
Martin PR, Hobbs M, Free PD, Jeske Y, Mattick JS
Mol Microbiol 1993 Aug;9(4):857-68
PubMed ID: 7901733
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344