Pseudomonas aeruginosa PAO1, PA5065 (ubiB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5065
Name
ubiB
Synonym: yigR
Synonym: aarF
Replicon chromosome
Genomic location 5704077 - 5705678 (+ strand)

Cross-References

RefSeq NP_253752.1
GI 15600258
Affymetrix PA5065_at
Entrez 878018
GenBank AAG08450.1
INSDC AAG08450.1
NCBI Locus Tag PA5065
protein_id(GenBank) gb|AAG08450.1|AE004919_11|gnl|PseudoCAP|PA5065
TIGR NTL03PA05066
UniParc UPI0000137989
UniProtKB Acc Q9HUB8
UniProtKB ID UBIB_PSEAE
UniRef100 UniRef100_Q9HUB8
UniRef50 UniRef50_O07443
UniRef90 UniRef90_Q9HUB8

Product

Feature Type CDS
Coding Frame 1
Product Name
ubiquinone biosynthetic protein UbiB
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (61.72 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 8.11
Kyte-Doolittle Hydrophobicity Value -0.129
Molecular Weight (kDa) 61.7
Isoelectric Point (pI) 9.44

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 494 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004666 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5065
Search term: ubiB

Human Homologs

Ensembl 110, assembly GRCh38.p14
aarF domain containing kinase 5 [Source:HGNC Symbol;Acc:HGNC:21738]
E-value: 2.2e-29
Percent Identity: 31.0
Ensembl 110, assembly GRCh38.p14
aarF domain containing kinase 5 [Source:HGNC Symbol;Acc:HGNC:21738]
E-value: 2.2e-29
Percent Identity: 31.0

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification of Escherichia coli ubiB, a gene required for the first monooxygenase step in ubiquinone biosynthesis.
Poon WW, Davis DE, Ha HT, Jonassen T, Rather PN, Clarke CF
J Bacteriol 2000 Sep;182(18):5139-46
PubMed ID: 10960098
Identification and characterization of aarF, a locus required for production of ubiquinone in Providencia stuartii and Escherichia coli and for expression of 2'-N-acetyltransferase in P. stuartii.
Macinga DR, Cook GM, Poole RK, Rather PN
J Bacteriol 1998 Jan;180(1):128-35
PubMed ID: 9422602
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344